In simple terms what is the difference between light cone quantisation and light front quantisation?

Susskind in his paper "The World as a Hologram" uses the term light front quantisation. I know only of light cone quantisation, hence this question. I am trying to see the connection between...

19 May 2020 832 3 View

Unknown bands pervasive in SDS-PAGE?

I am having problems with some samples in SDS-PAGE. For 2 specific proteins (one viral protein one serum protein) I am getting almost consistent unexpected bands across the gel. I have included...

05 August 2019 3,635 1 View

Dissolving BSA into PBS?

I had created a 1x stock of PBS (250ml) and had tried to 1.8g of Bovine Albumin Fraction V into it. For some reason I am unable to successfully get it to dissolve. I have tried at 4*C and at 37*C...

30 July 2019 3,638 1 View

Radiation treatment of polymers?

Hello! I would like to carry out some experimentation of radiation treatment of polymers and its effects on the mechanical properties (depending on exposure rate and type of radiation). Is there...

17 June 2019 2,568 7 View

DNA Smearing (2% Agarose Gel)?

Hello, I've had some trouble with my gels, and are pretty much clueless on what to try next. I've troubleshooted this for the last month to no avail and would like to get some input from the...

02 June 2019 4,327 3 View

Does overexpression of proteins in vitro affect quality of the final product?

I have read some papers concerning expression levels of proteins and instances of misfolding to due to "back up" during cellular processing. I am expressing viral surface proteins and derived...

11 April 2019 5,397 8 View

HPLC amino acid analysis with cell culture?

I'm trying to analyze the amino acid composition of L6 muscle cells with the use of HPLC. How can I go about harvesting and preparing my samples for HPLC use.

15 June 2018 8,667 2 View

Invasive devices are being used to stimulate organs in the body. What frequencies, voltages, and pulse lengths are being used?

There are a lot of invasive technologies in use today that are used to stimulate organs, the nervous system, the brain, and pain receptors in the body. One example, would be a device that...

03 March 2018 570 3 View

How can I generate parameters for modified short peptides for its molecular dynamic simulations in Gromacs?

I am doing the coputation analysis of modified short peptides in AD. But I have no idea how to generate parameters for modified peptides. Any help will be highly appereciated.

13 February 2018 5,240 4 View

Can anyone share a protocol to separate insect fatbody cells from hemolymph, without breaking the delicate fatbody cells?

I'm working with psyllids, and I'm trying to extract only fat body cells, free of hemolymph. Alternately, I'm looking for a method to separate the two, perhaps by centrifugation. The end goal is...

31 July 2017 3,492 2 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

What element is driving the different slop in the low frequency tail?

My study consists simple corrosion inhibitors in salty brine. Right now I just fit my circuit with randles with CPE. Just wondering how the slop can given more info of the experiment. Or any...

01 March 2021 6,474 3 View

Can I use a protein-devoid condition as my negative control in ELISA?

Hello, I am running a sandwich-type ELISA where I am trying to inhibit a protein-protein interaction using small molecules. For each experiment, I determine my Z' (Z-prime) to determine the...

23 February 2021 7,429 1 View

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23 February 2021 4,014 3 View

How to reduce static electricity of powder after lyophilization?

I am working on synthesizing and then purifying peptides using the SPPS system. After purification of the peptide, the peptide powder is obtained using a freeze dryer. When measuring the powder,...

22 February 2021 10,099 5 View

Can we remove the fmoc group after peptide cleavage?

I have synthesized the peptide using Fmoc based solid phase peptide synthesis. Also I have cleaved the peptide from the resin and lyophilized it, but later realized that, I forgot to deprotect the...

21 February 2021 4,537 3 View

Did anyone perform CD107a degranulation assay without the use of inhibitors like monensin?

I am planning to perform a degranulation assay using CD107a antibody. Some resources suggest to use inhibitors like monensin to inhibit the intracellular traficking and acidification of the...

21 February 2021 6,260 3 View

How T cells recognise non-peptide antigens?

This might sound very basic question, but please share with me an explanation of how the T cell is activated when the antigen is not a peptide? What happens? And what are the molecules involved in...

21 February 2021 564 3 View

Phenol-pH graphic?

We want to use phenol pH graphic to measure the pH of mediums before and after inhibitor application to cells. We have phenol absorbence values at 460, 560 and 720 nm wavelength. When we measure...

20 February 2021 7,700 3 View

Which way will be better to give OGT inhibitors doses through intravenous or orally for pregnant female rats and concentration suggestion?

I'm planning to use ST078925 OGT inhibitor from TimTec (OGT-O-linked N-acetylglucosamine (GlcNAc) transferase) for female rats.

16 February 2021 7,850 2 View