Peptides as multifunctional inhibitors of AD.
I was left with this question after reading about increased aerobic capacity conferred via fecal/microbiome replacement after exercise in humans (endurance athletes) and mice. Can we conclude the...
02 May 2023 1,874 1 View
I'm getting very low to no yield using this kit. Has anyone used this kit successfully, and if so, are there are modifications or suggestions you used to improve your yield? Thanks!
01 December 2021 1,908 1 View
I want to centrifuge yogurt in order to pellet bacteria and yeast cells. But of course all the fat pellets as well. I tried a mixture of EDTA and TE buffer as used in a journal article I found,...
21 November 2021 5,164 4 View
Hi, my name is Sarah-Therese Mann and I am a Master's student at the UIB in Spain. I am conducting a study about eye-witness memory and language. Therefore I am looking for native speakers of...
18 March 2021 496 4 View
Susskind in his paper "The World as a Hologram" uses the term light front quantisation. I know only of light cone quantisation, hence this question. I am trying to see the connection between...
19 May 2020 993 3 View
In running camera traps on Chuihuahuan desert water cachements, I have noticed that Bobwhite quail do not show themselves watering on camera until late autumn, when the Shinnery oak have dropped...
21 December 2019 4,172 1 View
I am wondering if anyone can explain some apparent weight discrepancies between SDS-PAGE and Blue Native PAGE. I understand that the underlying principle is different in each, which may produce...
22 September 2019 1,014 2 View
I am having problems with some samples in SDS-PAGE. For 2 specific proteins (one viral protein one serum protein) I am getting almost consistent unexpected bands across the gel. I have included...
05 August 2019 3,781 1 View
I had created a 1x stock of PBS (250ml) and had tried to 1.8g of Bovine Albumin Fraction V into it. For some reason I am unable to successfully get it to dissolve. I have tried at 4*C and at 37*C...
30 July 2019 3,748 1 View
I am curious as to the particular mechanisms by which exogenous antibody can induce protection. Considering these antibodies do not result in natural immunity, do they have limited functions in...
09 July 2019 1,570 2 View
We intend to study the interaction between peptides and polymer (like PP, PE and PS) through MD simulations using Martini force fields ( Martini 2 for PP and Martini 3 for PE, PS). We have...
08 August 2024 4,842 0 View
Hi, we have measured tryptic peptides using both DDA and DIA method on QExactive. In DDA replicates i saw unusual intensity drops occurring at the same sections of chromatograms in DDA replicates...
07 August 2024 3,218 4 View
Hello, I am currently analyzing some phosphoproteomics data, but I have peptides with multiple phosphorylation sites or phosphorylations together with carbamidomethylation or oxidation. How can I...
04 August 2024 8,432 3 View
Recently I'm trying to use Sep-pack C18 1 cc Vac Cartridge (Waters: SKU: WAT054955) to desalt digested peptides. However, the recovery rate is usually 40-60%. What is the normal recovery rate?
31 July 2024 6,544 11 View
I am currently working on a project that involves extracting cell membranes, for which we disrupt the cells using sonication. During the initial extraction process, we add protease inhibitors to...
30 July 2024 7,077 1 View
So i have a few peptides (synthesized by SPPS and not cleaved from resin) and I want to add a maleimide label. We have NHS-PEG-Maleimide and from literature it is evident that the free amine group...
18 July 2024 4,075 3 View
Hello everyone! I am currently working on the expression and purification of an antimicrobial peptide that experiences reduced activity after each freeze-thaw cycle. To improve its stability, I...
10 July 2024 6,830 1 View
Recently I looked up a literature《A Glucose-6-Phosphate Isomerase Peptide Induces T and BCell-Dependent Chronic Arthritis in C57BL/10 Mice Arthritis without Reactive Oxygen Species and...
09 July 2024 6,657 0 View
There are three factors involved in the activity, the substrate acting as a substrate, the substrate acting as an inhibitor and another inhibitor for which you want to measure the Ki.
08 July 2024 4,988 1 View
I am working on my PTM project. I have some peptide. I got the result of doing MD of those peptide without PTM in different force field like Charm-GUI, Amber, Gromacs. Now I have to do MD of those...
05 July 2024 9,346 0 View
