How to help ToxTrac to detect an moving animal?

Hi, I would like to use ToxTrac to help me with my hornet's videos. I did 600 experiments in an olfactometer to follow the behavior of hornets. They are pretty "big" but ToxTarc never detected...

23 August 2020 8,716 4 View

Survey template to assess added value in facility management?

The survey instrument is needed to assess how a hospital construction organisation perceives added value to the customer and users in strategically managing the build.

26 December 2018 221 3 View

Residual analysis after excat Fisher test (vs. Chi-square)?

I am working with small sample size qualitative clinical data (use attach file) In this context I have used a exact Fisher test as some expected frequency < 5. More exactly I perform a perform...

28 November 2018 9,832 2 View

Transwell Migration assay ?

Dear Colleagues I am bout to explore the extravasation of tumours by in vitro modelling using a trans well (Boyden chamber) assay; I must confess this is completely new to me In essence, we are...

07 May 2017 8,296 3 View

Standard curves in qPCR for very low copy number genes ?

Dear Colleague(s) I am dealing with a gene at the moment that exhibits very low expression and is barely detectable from neat cDNA. As such if you dilute the cDNA to construct a standard curve...

01 June 2016 4,189 7 View

REST Gene expression software: Windows compatibility issues ?

23 March 2016 6,328 6 View

Housekeeper choice for real time qPCR using human heart RNA ?

Dear Colleague(s) I am well versed with real time qPCR but have never actually worked with human heart tissue to determine relative expression of a GOI, viz cytochrome p450 2J2 variant. In...

14 January 2016 2,996 2 View

Does anyone know what the composition is of the detection reagent B in the ELISA Kit for alanine transaminase?

08 March 2015 6,423 3 View

Does anyone know if you can perform delta delta Ct analysis on gel based bands ?

Hi, I routinely measure gene expression by qPCR using the delta delta ct method, wherein a test gene is measured in treated and untreated samples and both are normalised (for loading/amplification...

20 October 2014 5,527 7 View

Measures of association vs predictive power

There is an excellent paper in the October issue of Annals of Epidemiology by Swaminathan et al that shows why measures of association cannot be used to quantity predictive power

01 January 1970 9,116 1 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

What are the new 2D nanomaterials were used for Silver nanoparticles stabilization?

In the stabilization of AgNPs are vital in current research interest. However, some of the 2D nano-materials like graphene oxide, MoS2, etc... previously used to stabilize it. But still they shown...

01 March 2021 2,207 3 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

How do I transfect a large plasmid into PC12 cells with nerve growth factor (NGF) receptor?

I have a large 16 kb plasmid, which I need to transfect into PC12 cells. Lipofectamin 2000 didn't work and with GFP alone the transfection rate is very low. I also tried the neon invitrogen...

25 February 2021 6,635 5 View

What software programs can directly simulate Additive Manufacturing Directed Energy Deposition (DED)?

I understand there are a lot of software programs that can simulate DED by going through specific modeling, simulation, and commands. For me, that was time consuming and results were not accurate....

24 February 2021 4,428 3 View

I am looking for a simple software program to model 3D chemical bonds. The purpose is to generate high-quality graphical abstracts. Any suggestion?

Hello everyone: I am looking for a simple software program to model chemical bonds. The purpose is to generate a high-quality graphical abstract for a top leading journal in the field of...

23 February 2021 8,012 3 View

Thermal reduction of graphene oxide.?

I am trying to reduce graphene oxide by thermally but I have no proper information regarding this. Can any one help me regarding the details about temperature and time for the reduction of...

23 February 2021 4,155 4 View

Is there an ideal incubation time of RGO-modified SPCE before subsequent use, i.e., electrochemical characterization or further modification?

I notice that upon washing and air-drying of my RGO/SPCE surface and characterizing again via DPV, the current response towards ferricyanide probe significantly decreases. My worry is that the...

22 February 2021 5,324 3 View

Which programs are to be used to register 2D image onto a 3D image data set?

I am trying to register a light micrograph onto a 3D CT data set, as I want to extract the corresponding image from the CT data set. Is there any software or program can do this accurately? I am...

21 February 2021 8,220 3 View

What is the better software for approximation of 3D surface with equations?

I have a massive of x, y, z coordinates of 2D surface in 3D space, which builded in Ansys HFSS or AutoCAD. How can I get the analitical aproximation of this surface by formulas?

21 February 2021 3,444 7 View