Anyone know what promoters would be good to use to knockdown or over express a gene in macrophages (invivo)?

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M. Zahied Johan

You can start by reading this paper http://www.ncbi.nlm.nih.gov/pubmed/22262441

Lukas Pawig

LysM-Cre could be useful for knockout in myeloid cells (i.e. macrophages and neutrophils).

http://www.ncbi.nlm.nih.gov/pubmed/10621974

Naveen Kumar Tangudu

Thanks a lot to M.Zahied!

Thanks Lukas, Did you use or have plasmid with CD68 promoter since LysMCre (I did use this promoter) could also delete gene in neutrophils, I would like to use a promoter with more specificity towards macrophages. Thanks Once again!!

Hey, no sorry, we only used the LysM model..

Ok, Thanks for your time!