I am wondering if anyone has used the pre-optimized primer pairs from Genecopoeia for RT-qPCR. I am considering these for some mouse transcripts.
Hello!
I did use them for two different transcripts and they worked fine.
Ornella
Thanks Ornella for your recommendation
I am doing a three point bend test with concrete specimen of dimension 280mm*90mm*90mm. I am putting 2 sensors near the notch with 3cms away from both the sides. the experiment ran for 20 mins but...
13 February 2021 2,990 1 View
I would like to use NIST test suite for Cryptographic Applications on a color image to test whether the image is random or not. But I don't know how to do all these tests. I used some...
06 April 2020 2,021 1 View
R and DEM (pysical model )?
13 February 2019 10,010 3 View
Which countrys should i talk about and what different topics
07 August 2018 1,799 3 View
I am doing PhD, and I need to create an LDPC codes for my initial understanding
03 February 2016 2,490 3 View
I'm planning to set-up an in-vitro osteoclastogenesis assay from mouse bone marrow culture, followed by TRAP staining. Could someone share a detailed, working protocol for that? Any suggestions...
06 July 2014 7,687 15 View
Many countries had their Universities classes interrupted due to covid 19 pandemia. Now we have to prepare ourselves to come back to our classes at University. Theoretical classes seem easier to...
01 January 1970 5,410 1 View
Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...
03 March 2021 687 1 View
I have a dataset with about 80 different species. As usual, some species are very easy to identify with certainty whereas others are more difficult, which means that I am less certain of my...
03 March 2021 8,066 4 View
Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...
02 March 2021 4,029 3 View
Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...
02 March 2021 2,616 1 View
If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis
02 March 2021 7,670 3 View
Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...
02 March 2021 4,569 4 View
Hello! I have a dataset of n=3000 nested within 8 countries with approximately 200 or 400 responses in each country. I originally planned to perform multilevel modelling with 4 dependent variables...
02 March 2021 6,865 1 View
how to calculate BEHAVIOURAL REGULATION IN EXERCISE QUESTIONNAIRE BREQ 3 ,which contain 24 questions and each question has 5 answers (0- Strongly disagree , 1-disagree, 2- Neither agree nor...
02 March 2021 811 3 View
So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...
02 March 2021 1,146 2 View
I am at the beginner level in drug design and drug discovery, and I am interested to learn more about this field. Which books are highly recommended to start from and gives me all essential...
02 March 2021 8,437 2 View