Anyone having experience with Genecopoeia All-in-One™ qPCR Primers for mouse transcripts?

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Hi all, could anyone please suggest me how to do Data Analysis of Cell Proliferation Assay using Flow Jo Software in Flow Cytometry ?

Its an In Vitro Cell Proliferation using Leukemia patient cells lines. Cell Proliferation Dye Used : Tag-It Violet Cell Proliferation Dye. Thank you a lot.

09 June 2024 1,194 1 View

Effects of Microplastics on mangroves plants and how mangrove plants consume microplastics?

Effect of Microplastics on the Germination and Growth of Mangrove Plants

17 September 2023 9,376 0 View

Modelling of Microbial fuel cells for maximum current density and substrate removal in MATLAB?

how to do modling and create code in MATLAB?

14 March 2023 5,703 3 View

How can I prepare 50 ug/mL of Phorbol Myristate Acetate (PMA) knowing that I have 1mg of PMA ? How much DMSO should I add ?

I want to differentiate THP1 cells into macrophages, but I'm lost with the calculation.

26 December 2022 503 1 View

Need sub scales of Connor-Davidson Resilience scale by Kathryn M. Connor and Jonathan R.T. Davidson?

Hello! I'm student of Bs(Hons) psychology from university of wah Pakistan. Currently I'm doing research to see the relationship of fear and death anxiety during COVID-19 and seeing moderating...

14 December 2021 819 3 View

Need sub scales of Revised Death Anxiety scale by James and Powell?

I'm student of Bs(Hons) psychology from university of Wah Pakistan. Currently I'm doing research to see the relationship of fear and death anxiety during COVID-19. I found revised death anxiety...

14 December 2021 2,655 2 View

'Co-citation analysis' OR 'Keyword co-occurrence analysis' - which one is better and effective to reflect thematic area of a research field?

Dear scholars, Bibliometric analysis is a very effective way of analyzing previous literature and development of any research field. But here my question is if I want to figure out the thematic...

10 October 2021 9,785 10 View

Why erythroblastosis fetalis is only result from incompatibility in Rh system but not ABO system?

normally it is caused when the fetus is Rh positive and the mother is Rh negative, but why it doesn't caused for example when the mother is B and the fetus is A?

07 September 2021 1,661 4 View

How to verify whether the Acoustic emission data obtained is correct and does not contain the noise data?

I am doing AE experiment in FRC concrete by keeping the threshold as 30 db (as low as possible to get maximum number of hits without noise). I want to verify whether the AE data obtained is...

05 March 2021 8,623 4 View

Receiving very less number of hits and events in acoustic emission tests?

I am doing a three point bend test with concrete specimen of dimension 280mm*90mm*90mm. I am putting 2 sensors near the notch with 3cms away from both the sides. the experiment ran for 20 mins but...

13 February 2021 3,285 1 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Research Methodology - Impact of Corporate Reputation on Stakeholders Behaviors?

Please can anyone support with the survey questions based on RQ measures and propose how to do it in FMCG industry and include as well the role of brand equity Thanks

06 August 2024 949 0 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

Please explain how the plastic input value should be considered from the true stress-strain curve for the bilinear elastoplastic material model ?

I am working on Abaqus/Explicit(Quasistatic ) for the deformation of the auxetic structure model. Please explain how the plastic input value should be considered from the true stress-strain curve...

05 August 2024 454 3 View

"A Markov-like Model for Patient Progression"?

A Markov-like Model for Patient Progression" Markov Chain Monte Carlo (MCMC) Markov Chain Monte Carlo (MCMC) is a powerful computational technique used to draw samples from a probability...

05 August 2024 10,079 0 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

Anyone having idea about VN primer for miRNA primer design ?

How to design VN primer to attach with universal reverse primer

05 August 2024 2,116 3 View

How to report results of Generalised Linear Mixed Models in a journal article?

Hi everyone, If you have written or come across any papers where Generalised Linear Mixed Models are used to examine intervention (e.g., in mental health) efficacy, could you please share the...

04 August 2024 4,130 4 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View