Anyone have IF protocol for anti-Staufen?

More Adrienne Anderson's questions See All

Help with bovine muscle histology?

Hello, I am freezing "normal" bovine muscle (literally it is steak I got from the grocery store), and the pathologist is telling me that there is freezing artifact. Looking at an H&E, there is...

11 December 2016 3,550 11 View

How do I get rid of background staining in IF?

Hello, I'm having a lot of problems trying a paladin IF stain on skeletal muscle tissue. My negative controls seem to show more staining than my slides. For the negative control, I'm just omitting...

10 November 2016 8,365 3 View

How do you stain cultured cells on coverslips?

My colleague grew some cells on coverslips for me to do FISH and IF staining. This is my first time staining this way. Can anyone share their method with me? What I mean specifically are the...

09 October 2016 2,757 5 View

Fluorescence staining only the periphery of tissue?

I've been working on some FISH/IHC combo stains which have been working well until this last run where I got bright staining on the edge of the tissues but very minimal staining in the middle. The...

08 September 2016 5,895 6 View

Antibody dilution/titration help?

Hello, I am about to titer an antibody (and I have little experience with this). If I received a stock concentration of 2 mg/mL and the recommended dilution for my work is 1:200.....is this 1:200...

08 September 2016 7,982 2 View

Does anyone do IF for the MBNL1 protein, and if so, can you recommend a good antibody and protocol for fresh frozen muscle biopsies?

I am using snap frozen muscle biopsies and am using a non-commercial antibody currently but would like to start buying a commercial antibody.

07 August 2016 6,630 0 View

Mouse CD3 antibody sequence?

Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much

08 August 2024 9,157 4 View

Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 505 3 View

Are there instances where molecules with larger molecular weights exhibit greater mobility than those with smaller molecular weights?

Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...

06 August 2024 1,495 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

The Curse of Evolution and Complexity?

Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...

05 August 2024 6,247 3 View

For an in-vitro drug release study, what molecular weight cut-off (MWCO) dialysis bag is required for a 117 kDa protein?

kindly reply me. Thanking you in advance.

05 August 2024 7,727 4 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to start a Molecular Dynamics Simulation?

Is it possible to conduct a molecular dynamics simulation to see the effects of a specific carbohydrate on the structure of lipids (e.g., micelle structure)? I am a beginner in this field and plan...

03 August 2024 3,371 3 View

Which will be the best software for the Hydration shell analysis with molecular dynamics?

I am using a windows system, what software I should use for hydration shell analysis with molecular dynamics?

02 August 2024 3,143 4 View

Can anyone provide me with molecular docking softwares/ websites?

Molecular docking software/ websites?

02 August 2024 8,704 7 View

Mushtaq Ahmad

Specific Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

http://www.abcam.com/staufen-antibody-epr7966-ab137100-protocols.html

Adrienne Anderson

Thank you!