Help with bovine muscle histology?

Hello, I am freezing "normal" bovine muscle (literally it is steak I got from the grocery store), and the pathologist is telling me that there is freezing artifact. Looking at an H&E, there is...

11 December 2016 3,400 11 View

How do I get rid of background staining in IF?

Hello, I'm having a lot of problems trying a paladin IF stain on skeletal muscle tissue. My negative controls seem to show more staining than my slides. For the negative control, I'm just omitting...

10 November 2016 8,270 3 View

How do you stain cultured cells on coverslips?

09 October 2016 2,681 5 View

Fluorescence staining only the periphery of tissue?

I've been working on some FISH/IHC combo stains which have been working well until this last run where I got bright staining on the edge of the tissues but very minimal staining in the middle. The...

08 September 2016 5,807 6 View

Antibody dilution/titration help?

Hello, I am about to titer an antibody (and I have little experience with this). If I received a stock concentration of 2 mg/mL and the recommended dilution for my work is 1:200.....is this 1:200...

08 September 2016 7,909 2 View

Anyone have IF protocol for anti-Staufen?

What is the best way to find an IF protocol? The website where I purchased the antibody (which is anti-Staufen) doesn't have any protocols. Thanks!

07 August 2016 7,130 2 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

Anyone know of a good progesterone antibody?

I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.

28 February 2021 5,929 2 View