I am using snap frozen muscle biopsies and am using a non-commercial antibody currently but would like to start buying a commercial antibody.
Hello, I am freezing "normal" bovine muscle (literally it is steak I got from the grocery store), and the pathologist is telling me that there is freezing artifact. Looking at an H&E, there is...
11 December 2016 3,550 11 View
Hello, I'm having a lot of problems trying a paladin IF stain on skeletal muscle tissue. My negative controls seem to show more staining than my slides. For the negative control, I'm just omitting...
10 November 2016 8,365 3 View
My colleague grew some cells on coverslips for me to do FISH and IF staining. This is my first time staining this way. Can anyone share their method with me? What I mean specifically are the...
09 October 2016 2,757 5 View
I've been working on some FISH/IHC combo stains which have been working well until this last run where I got bright staining on the edge of the tissues but very minimal staining in the middle. The...
08 September 2016 5,895 6 View
Hello, I am about to titer an antibody (and I have little experience with this). If I received a stock concentration of 2 mg/mL and the recommended dilution for my work is 1:200.....is this 1:200...
08 September 2016 7,982 2 View
What is the best way to find an IF protocol? The website where I purchased the antibody (which is anti-Staufen) doesn't have any protocols. Thanks!
07 August 2016 7,216 2 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
When the entire neocortex is ablated in rodents, although they are still able to swim, all the limbs move continuously and asynchronously (Vanderwolf 2006; Vanderwolf et al. 1978). Normal animals...
03 August 2024 835 3 View
We have a lateral flow test on Sartorius 140 NC. The conjugate is gold-monoclonal antibody. Using different control lines (GAM, protein A, protein G) we get a very strong leading edge with reduced...
31 July 2024 4,510 3 View
What is the thermal expansion coefficient of human muscle?
27 July 2024 7,190 0 View
I am struggling to find a seeding density that will allow my cells to not aggregate together. Anything helps!
24 July 2024 3,460 1 View
I am performing IgG purification and I have to show my results on SDS-PAGE. I use 10% tris glycine gel and prepare the samples under non reducing conditions. I am new to antibodies and therefore...
23 July 2024 6,664 6 View
I have immunized BalB/C mice with a protein using the intradermal (ID) method with Complete Freund's Adjuvant (CFA) and Incomplete Freund's Adjuvant (IFA), following a 14-day interval and three...
22 July 2024 9,160 2 View
Hello, I recently stained my cells with an Arf6 antibody. The healthy control looks pretty normal, while the disease group cells have abnormally large black structures in the cell body. Has anyone...
21 July 2024 1,044 1 View
I founde some textbooks stating that typical skeletal muscles can shorten up to 70% (optimally up to 30%) during contraction. However, I would like to look at muscles per region. This might seem...
21 July 2024 399 3 View