Adrienne Anderson

7 Questions 14 Answers 0 Followers

Questions related from Adrienne Anderson

Help with bovine muscle histology?

Hello, I am freezing "normal" bovine muscle (literally it is steak I got from the grocery store), and the pathologist is telling me that there is freezing artifact. Looking at an H&E, there is...

12 December 2016 3,551 11 View

How do I get rid of background staining in IF?

Hello, I'm having a lot of problems trying a paladin IF stain on skeletal muscle tissue. My negative controls seem to show more staining than my slides. For the negative control, I'm just omitting...

11 November 2016 8,366 3 View

How do you stain cultured cells on coverslips?

My colleague grew some cells on coverslips for me to do FISH and IF staining. This is my first time staining this way. Can anyone share their method with me? What I mean specifically are the...

10 October 2016 2,758 5 View

Antibody dilution/titration help?

Hello, I am about to titer an antibody (and I have little experience with this). If I received a stock concentration of 2 mg/mL and the recommended dilution for my work is 1:200.....is this 1:200...

09 September 2016 7,983 2 View

Fluorescence staining only the periphery of tissue?

I've been working on some FISH/IHC combo stains which have been working well until this last run where I got bright staining on the edge of the tissues but very minimal staining in the middle. The...

09 September 2016 5,897 6 View

Does anyone do IF for the MBNL1 protein, and if so, can you recommend a good antibody and protocol for fresh frozen muscle biopsies?

I am using snap frozen muscle biopsies and am using a non-commercial antibody currently but would like to start buying a commercial antibody.

08 August 2016 6,632 0 View

Anyone have IF protocol for anti-Staufen?

What is the best way to find an IF protocol? The website where I purchased the antibody (which is anti-Staufen) doesn't have any protocols. Thanks!

08 August 2016 7,217 2 View