If watercrystal damage happened in my whole brain samples, would there be any significant differences in the result of my immunoblotting between step A and step B (which are as follows)?
Step A: homogenise the whole brain and take the lysate for analysis
Step B: separate different neural cells (maybe by FACS) and take the lysate of different fractions for analysis
(*the protein targets are the same in both steps)
Stefan Kurtenbach
I don't know if I understand "water crystal damage", do you mean your samples froze? If your tissue froze than I would think that you cannot do the FACS sorting any more, as cells are damaged... You should still be fine homogenizing it and use it for e.g. western blots or other things.
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