Stefan Kurtenbach

6 Questions 11 Answers 0 Followers

Questions related from Stefan Kurtenbach

piRNA yRNA locations in the human genome?

Where can I find a BED file (or any other file) with all chromosomal locations of piRNAs and yRNAs in the human genome? Thanks!

07 March 2019 5,192 1 View

Is MACS2 callpeak output normalized?

I ChIpped RNApol2 in the same cells with two different treatment conditions. Using the respective input controls I used MACS2 callpeak to generate the bedfiles. Are these automatically normalized...

27 April 2016 4,031 2 View

No RNA after blood plasma ultracentrifugation of exosomes?

I'd be very grateful for some ideas... Blood plasma was generated by differential centrifugation (200g, 2000g, and 16.000g for 15 min). Frozen at -80deg for a few days. After thawing, plasma was...

14 April 2016 1,710 4 View

Can anyone recommend a protocol to isolate pure melanocytes from small human skin biopsies?

Does anyone have advice?

21 April 2014 10,044 2 View

Test if cells burst?

I want to shake cells to mechanically stimulate ATP release, but am afraid that some cells will burst. Is there a good test to check the supernatant afterwards for that?

03 December 2013 6,369 5 View

Color changing, reversible oxygen indicator (liquid)?

Does anyone know one? I'm planning seed cells in 96 well plates and perform a hypoxia measurement in a 96 well reader. Would be nice to have a liquid oxygen indicator which I would measure at the...

29 November 2013 9,737 1 View