Anyone has information about Trihalo compound and stain free gel?

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Ute Neubacher

Here is a link to Bio-Rad: https://www.bio-rad.com/de-de/applications-technologies/stain-free-imaging-technology?ID=NZ0G1815

May be it offers you all the informations you need.

Lan Zhu

Thank you Ute for your answer. I did come to know that we can make stain free gel in the lab from this website, but they don't have more information about their proprietary trihalo compound.

Anyway, I did find some useful papers, if anyone wants to try it out:

Ladner, C. L.; Yang, J.; Turner, R. J.; Edwards, R. A. Visible fluorescent detection of proteins in polyacrylamide gels without staining. Anal. Biochem. 2004, 326 (1), 13–20.

Edwards, R. A.; Jickling, G.; Turner, R. J. The Light-induced Reactions of Tryptophan with Halocompounds¶. Photochem. Photobiol. 2007, 75 (4), 362–368.

Rivero-Gutiérrez, B.; Anzola, A.; Martínez-Augustin, O.; De Medina, F. S. Stain-free detection as loading control alternative to Ponceau and housekeeping protein immunodetection in Western blotting. Anal. Biochem. 2014, 467, 1–3.

Ladner, C. L.; Tran, K.; Le, M.; Turner, R. J.; Edwards, R. A. Excited State Photoreaction between the Indole Side Chain of Tryptophan and Halocompounds Generates New Fluorophores and Unique Modifications. Photochem. Photobiol. 2014, 90 (5), 1027–1033.

Pristov, J. B.; Opačić, M.; Dimitrijević, M.; Babić, N.; Spasojević, I. A method for in-gel fluorescent visualization of proteins after native and sodium dodecyl sulfate polyacrylamide gel electrophoresis. Anal. Biochem. 2015, 480, 6–10.

Holzmüller, W.; Kulozik, U. Protein quantification by means of a stain-free SDS-PAGE technology without the need for analytical standards: Verification and validation of the method. J. Food Compos. Anal. 2016, 48, 128–134.

Ladner-Keay, C. L.; Turner, R. J.; Edwards, R. A. Fluorescent protein visualization immediately after gel electrophoresis using an in-gel trichloroethanol photoreaction with tryptophan. In Methods in Molecular Biology; Humana Press Inc., 2018; Bd. 1853, S 179–190.

Chopra, A.; Willmore, W. G.; Biggar, K. K. Protein quantification and visualization via ultraviolet-dependent labeling with 2,2,2-trichloroethanol. Sci. Rep. 2019, 9 (1), 1–8.

Carlos Martin de Hijas

Hi Lan, I believe that you are right and it is 2,2,2-trichloroethanol, as it is listed in their US SDS document: https://bio-rad-sds.thewercs.com/DirectDocumentDownloader/Document?prd=HRLS00769~~PDF~~MTR~~AGHS~~EN

Sebastien LEON

You can check this patent which I think corresponds to this: https://patents.google.com/patent/WO2013163451A1/en