Good morning, I am going to start culturing PBMCs with TexMacs Medium, T cell transact and IL-2 (30 IU/ml). Anyone has tricks and tips about this process or has ever tried it with the t cell transact? I am going to start from cryopreseerved PBMCs and I am not going to isolate them, at the end I am hoping to have a large amount of t-cells since the activation is going to be done with CD3 and CD28 (T cell transact).

Thank you in advance!

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