Hello Amazing researchers
Anyone ever did the RT-qPCR or made the cDNA from 384-well plate? I need to do RT-qPCR to evaluate the knock-down efficiency of esiRNA. But due to limited esiRNA, we need to do 384-well plate transfection. I am planning to use cell to ct kits, but was not sure if cells that are in 384-well plate would be enough? If someone has experience, can you please let me know if it worked and what were things to do? If possible, can someone share their protocol as well?
I have never done qPCR from 384 well plates, but with very low expression genes, or low quantities of nucleic acid in general, preamplification is a fairly well-validated and successful approach. There are examples in literature of both global and targeted preamplification prior to qPCR.
Hi,
I cannot give you the info for Cell to Ct Kits, but a similar approach would be fastlane potentially as one-Step PCR. This sounds like a good approach as it works with limited material.
Good luck
Sven
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