Hello,
are there any murine cancer cell lines out there that once injected subcu will metastasize to the liver? I know there is LLC and some melanoma cell lines that will go to the lung. Appreciate any input!
thanks
Dear Christof!
Please You read this article
https://link.springer.com/article/10.1007/s12253-020-00805-3#citeas
Comparison of Different Colorectal Cancer With Liver Metastases Models Using Six Colorectal Cancer Cell Lines (attached)
Cell Culture Six generally available human CRC cell lines, SW620, SW480, HCT116, HT29, LOVO and DLD1,
Fig. 1 Bioluminescence monitors tumor burden growth and liver metastases in the orthotopic transplantation group
Fig. 2 Liver metastases in the ectopic transplantation group. a The number of animals proceed to develop into liver metastases via six CRC cell lines. b
Article Establishment of Peritoneal and Hepatic Metastasis Mouse Xen...
Mouse peritoneal metastasis models. MKN1-Luc, N87, KATO III, MKN45-Luc, NUGC4, and OCUM-1
Mouse hepatic metastasis models. Under general anesthesia, we mobilized and excised the spleens of nude mouse, which were then subcutaneously implanted. A total of 0.5×106 MKN45-Luc cells were suspended in 100 μl of PBS and injected into the subcutaneously implanted spleens. For direct injection into the portal vein, nude mice and NOD/SCID mice were placed under general anesthesia and laparotomized. Then, 1.0×106 MKN45-Luc and 0.5×106 MKN1-Luc cells were suspended in 100 μl of PBS and directly injected into the portal veins of mice using a 35-gauge NanoNeedle (NIPPON Genetics, Tokyo, Japan). After injection of the cell suspensions, we oppressed the puncture site of the portal vein using SURGICEL (Johnson & Johnson, NJ, USA) for a few minutes. Twelve weeks after injection, these mice were killed and hepatic metastasis formation was observed under direct viewing.
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