Lipid micromixer for lipid nanoparticle?

I am making lipid nanoparticles for DNA encapsulation, the device for it is too expensive, I would like to get a lipid micromixer for making lipid nanoparticle, any body know where to buy, which...

06 July 2019 4,746 3 View

Looking for a neural stem cell line ?

I need a neural stem/progenitor cell line to test drug toxicity, which one is easy to culture and differentiate ?

01 January 2018 5,206 4 View

Looking for a neural stem cell line ?

I need a neural stem/progenitor cell line to test drug toxicity, which one is easy to culture and differentiate ?

31 December 2017 7,239 5 View

ATCC cell line contamination

recently obtained a cell lines from ATCC, directly thaw and plated in DMEM+10%FBS, 5 days later both were contaminated, probably by fungi, in both flasks, medium was good because other cell...

01 January 1970 6,966 10 View

Crispr/Cas9 gene knock-out

I am trying to KO a gene in liver as well in cell line, using 3 sgRNA cloned into an all-in-one lentivirus with cas9 in it, but somehow I did not see any expression change, any suggestion?

01 January 1970 4,432 6 View

ATCC cell line contamination

recently obtained a cell lines from ATCC, directly thaw and plated in DMEM+10%FBS, 5 days later both were contaminated, probably by fungi, in both flasks, medium was good because other cell...

01 January 1970 9,439 5 View

Crispr/Cas9 gene knock-out

I am trying to KO a gene in liver as well in cell line, using 3 sgRNA cloned into an all-in-one lentivirus with cas9 in it, but somehow I did not see any expression change, any suggestion?

01 January 1970 9,167 6 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

No title

02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View