Peter Dd

8 Questions 17 Answers 0 Followers

Questions related from Peter Dd

Lipid micromixer for lipid nanoparticle?

I am making lipid nanoparticles for DNA encapsulation, the device for it is too expensive, I would like to get a lipid micromixer for making lipid nanoparticle, any body know where to buy, which...

07 July 2019 4,750 3 View

Looking for a neural stem cell line ?

I need a neural stem/progenitor cell line to test drug toxicity, which one is easy to culture and differentiate ?

02 January 2018 5,223 4 View

Looking for a neural stem cell line ?

I need a neural stem/progenitor cell line to test drug toxicity, which one is easy to culture and differentiate ?

01 January 2018 7,245 5 View

Anybody used ATCC ACS-5003 Neural Progenitor Cell ?

it is premature cell line, the reference listed under product is about other thing, It was very difficult to maintain regular stem cell growth, any suggestion?

12 December 2017 6,625 0 View

Crispr/Cas9 gene knock-out

I am trying to KO a gene in liver as well in cell line, using 3 sgRNA cloned into an all-in-one lentivirus with cas9 in it, but somehow I did not see any expression change, any suggestion?

01 January 1970 9,187 6 View

ATCC cell line contamination

recently obtained a cell lines from ATCC, directly thaw and plated in DMEM+10%FBS, 5 days later both were contaminated, probably by fungi, in both flasks, medium was good because other cell...

01 January 1970 9,444 5 View

Crispr/Cas9 gene knock-out

I am trying to KO a gene in liver as well in cell line, using 3 sgRNA cloned into an all-in-one lentivirus with cas9 in it, but somehow I did not see any expression change, any suggestion?

01 January 1970 4,440 6 View

ATCC cell line contamination

recently obtained a cell lines from ATCC, directly thaw and plated in DMEM+10%FBS, 5 days later both were contaminated, probably by fungi, in both flasks, medium was good because other cell...

01 January 1970 6,972 10 View