you can find the protocol here
http://www.currentprotocols.com/WileyCDA/CPUnit/refId-mb0107.html
"Alternate Protocol 3: Plasmid DNA Purification by PEG Precipitation
Additional Materials (also see Basic Protocol 2)
Glucose/Tris/EDTA solution (UNIT 1.6)
0.2 M NaOH/1% (w/v) SDS [prepare fresh from 10 M NaOH and 10% (w/v) SDS stocks]
10 mg/ml DNase-free RNase (UNIT 3.13)
3 M potassium acetate solution, pH ~5.5 (see recipe)
Buffered phenol (UNIT 2.1A)
24:1 (v/v) chloroform/isoamyl alcohol (UNIT 2.1A)
10 M ammonium acetate (APPENDIX 2)
PEG solution (see recipe)
3 M sodium acetate, pH 5.5 (APPENDIX 2)
Sorvall SS-34 or Beckman JA-17 rotor (or equivalent)
Sorvall HB-4 rotor
"
variants of the protocol are available using a search engine of your choice.
This can be optimised for certain sources or downstream application.
As for the variant of the PEG i might suggest 8000 but you can possibly make do with 6000 as well.
Why do you want to use PEG when 150 mM NaCl and 2.5 volumes of 100% ethanol work perfectly?
You can check our article
Anal Biochem. 2008 Sep 15;380(2):310-4. doi: 10.1016/j.ab.2008.05.044. Epub 2008 Jun 3.
Thank you...all of you..I did this..and it is such a wonderful method to get rid of all sort of contamination.
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