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Questions related from Panchsheela Nogia
We are trying to prepare continuous/discontinuous sucrose gradient for isolating chloroplast membranes. Can anyone suggest which is the best gradient maker (which company) or can we prepare the...
01 January 2017 3,321 4 View
I have tried transforming A. thaliana and N. tabacum plants using Agrobacterium mediated co-cultivation/culture method but in case of A. thaliana, I could not get any callus after agroinfection...
10 October 2016 4,706 19 View
For GFP localization study by confocal microscopy, I need to prepare a permanent slide of the protoplast samples which are prepared from the agroinfiltrated leaves of Nicotiana benthamiana. Is...
07 July 2016 5,919 7 View
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04 April 2014 5,122 5 View
What are the important facts/requirements that we need to focus while growing A.thaliana in lab conditions?
06 June 2013 3,361 4 View
In this vector our gene will be under the control of lac promoter, so will it be active in plant system? Anybody have experience working with this vector?
05 May 2013 4,632 9 View
I want to clone a gene bw NcoI and SpeI sites
05 May 2013 5,609 3 View
Can we use PEG 6000 for this purpose?
04 April 2013 9,839 5 View
I am trying to express a hydrophobic membrane protein in N. benthamiana plant but the expression level is very low (checked with microscopy) so I doubt may be I need to modify the transformation...
01 January 1970 7,457 2 View
