I need to do analysis for following Dye and pigment samples by LCMS .
1]Reactive orange 131 Dye
2]Red 49K
3]Reactive Red 241
4]Yellow 9K
Why? Dyes and pigments are visible, thus use a PDA or DAD, not an MS (it's like using a shotgun to kill a fly). There are 'many' chromatographic conditions on the internet (try Google).
P.S. Most dyes and pigments are 'water' loving and elute very quickly in a chromatogram (even in the injection front).
Dear Bruce Neagle and Neha Patel
Thank you very much for your answer I am specific to my question.
I need to analyze for following Dye and pigment samples by LCMS do you have any article or method of LCMS . if so you could please share with me.
1]Reactive orange 131 Dye
2]Red 49K
3]Reactive Red 241
4]Yellow 9K
I have an access for LCMS system .
Regards
Review the Thermo Scientific website. They have chromatographic methods for dyes. Because they are 'water loving' I would use HILIC 1st, but it takes ~20 (not 10) column volumes to equilibrate the column.
I would review these papers;
Article Simultaneous determination of fifteen illegal dyes in animal...
Article Determination of 23 Dyes in Chili Powder and Paste by High-P...
Article Development of a method for the analysis of seven banned azo...
- Badges
- Science topic
- Similar topics
- Chemistry
- Biochemistry
- Biochemical Techniques
- Blotting Techniques
- Immunoblotting
- Dyes