Hello,
I am searching for a protocol to create a immobilized melibiose column to purify anti-alpha-Gal antibodies from human serum. the crosslinked guar gum column described here Article Preparation and property of crosslinking guar gum
takes a long time and at the end i do not elute any antibody using Glycine-HCL. I also tried method fromArticle Low reactivity of tumor MUC1-binding natural anti-α-galactos...
still no antibody. I am looking for another protocol to immobilize melibiose on to a poly-acrylamide or similar gel based on sugar-PAA conjugates mentioned hereArticle Neoglycoconjugates: Trade and art
. i could not get the protocol anywhere, anyone has tried to synthesize and is willing to share the protocol.- Badges
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- Biological Science
- Biochemistry
- Enzymology
- Enzymes
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