How to check the pH of E. coli culture initially? and how to change the pH of the E. coli culture?

I am working on E. coli 15597 prepared in Luria broth. Initially, the pH of LB is 7 after the inoculation of the E. coli it changes to 8.26 is it correct? can bacteria be alkaline?

15 October 2020 2,042 2 View

What are the best pipleline available for the RNA-Seq data analysis ?

What is the latest pipeline to run the RNA-Seq genomic data ?

12 October 2020 7,618 2 View

If an article is unknowingly published in a fake journal, is it considered as published or can the article be sent to some other journal to publish?

How do we handle this menace of fake journals?

14 July 2020 4,835 7 View

How do we differentiate conservation agriculture and regenerative agriculture in context of sustainable farming practice?

Sustainable farming practices appears into different names confusing the global audience which to follow and why? What makes conservation agriculture as different approach than regenerative...

30 June 2020 7,726 2 View

How to get DOI for my published article in a journal which has not given any DOI?

I want to know if Research Gate generates DOI for articles which has been published but has not been provided any DOI by the publisher?

23 May 2020 510 7 View

How many times do we have to check and report model fit measures during SEM?

I am trying to run a SEM which has four latent variables in it. Let us consider these variables as A, B, C and D. All are latent. Here A and B are independent variables, C is mediator and E is...

17 May 2020 4,091 3 View

What is the criteria to calculat e the plagarism?

What is the criteria to calculat plagarism for writing manuscript

06 April 2020 6,936 3 View

What happens if we lower the number of cycles in explicit dynamics for ansys ?

Hi I am a student pursing my final year in mechanical engineering. I am doing crash analysis of the car frame in Ansys software in explicit dynamics module. I had my analysis running for 2-3 days...

18 March 2020 3,709 3 View

Why contaminant is transporting from lower part to higher part in various column experiments? What is reason behind this?

can we transport the contaminant from the higher part to the lower part of the column?

25 December 2019 7,124 3 View

Can authentication in IoT be implemented using BlockChain techniques?

Authentication in IoT systems are vastly insecure. Mostly the problems lies on its open ended and probable untrustworthy nodes. Also, the need for a lighter authentication mechanism will always be...

09 December 2019 4,896 4 View

Could you recommend any books about Number Theory?

Beginner level books. High level books. Analytical Number Theory. Algebraic Number Theory. Combinatorical Number Theory. Books about partitions. Books about primes.

03 March 2021 7,174 5 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

What kind of intervention will be relevant to increase motivation among young sport trainees?

Any suggestion on what book to read is also welcome.

03 March 2021 1,646 4 View

I need to image mesoporous silica nanoparticles that are in ~100 nm size and do high resolution TEM to look at mesopores. what grids should I use?

Need to image mesoporous silica nanoparticles using the TEM. Also, need high resolution TEM images to see the mesoporous structure. Kindly suggest what kind of grids to use. Thanks, Shatadru

02 March 2021 1,787 2 View

How the parameters (such as Mw, Chemical bonds, polymer bland and so on) affect the mechanic property of polymer (such as storage, loss modulus) ?

Anyone can recommend any books on such topics? Thanks in advance.

02 March 2021 8,457 2 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

I am interested in medicinal chemistry field, which books are highly recommended to learn from?

I am at the beginner level in drug design and drug discovery, and I am interested to learn more about this field. Which books are highly recommended to start from and gives me all essential...

02 March 2021 8,437 2 View

Please suggest a good text book for "Research Methodology in Life Sciences". Which book would be good for PhD Scholars?

Please provide Book Title and author name

02 March 2021 9,059 3 View

Which is a lucid standard comprehensive book to guide on psychological scale construction and its standardization?

02 March 2021 7,082 3 View

What element is driving the different slop in the low frequency tail?

My study consists simple corrosion inhibitors in salty brine. Right now I just fit my circuit with randles with CPE. Just wondering how the slop can given more info of the experiment. Or any...

01 March 2021 6,474 3 View