I have recently conducted digestion experiments using Cas12a (LbCas12a, New England Biolabs) and the designed gRNA for a target sequence. The results in the agarose gel confirmed the presence of both a shorter band and the regular band, indicating that the cas12a is recognizing and cleaving the target sequence. However, despite several attempts, I have been unable to achieve the trans-cleavage activity of the ssDNA reporter by Cas12a. I have tried digesting the ssDNA reporter at 37°C for up to 2 hours, but the cleavage does not occur.