Any suggestions on the overexpression of GABA NT?

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Someone have the key or the installer of the program Mx pro 3000 (PCR real time)?

I need to install this program

25 July 2024 4,756 0 View

Which commercial technology is available ? 4 brain ?

Following https://catniplab.github.io/Tafazoli-LearningControlBrain/ and https://insights.princeton.edu/2020/05/learning-to-control-the-brain/ which commercial technology is available ? Probably...

15 July 2024 4,339 0 View

What could be causing these smears in my PCR electrophoresis gel?

I am new to running PCR gels. I loaded this gel and I thought it was fine, meaning I saw/felt no apparent punctures or spillovers to neighboring wells (see picture 1). When the gel started to run,...

30 June 2024 4,107 4 View

What are those white spots at the end?

I'been seeing those white spots at the end of my wet-transfer lately. Since the Ponceu Red doesn't stain I guess there isn't any protein. Since it's under the 42kDa mark It isn't a problem since I...

18 June 2024 1,734 0 View

Condensation of a Q8 serendipity FE to a Q4 FE with drilling DOFs?

Hello, Can somebody please give me some reference to a paper or book where it is explained how to condensate: * from a Q8 serendipity FE to a Q4 FE with drilling DOFs (16 DOFs to 12 DOFs). *...

05 June 2024 936 1 View

Organoid with Claustro can be linked to LLM like chatGPT ?

Organoid with Claustro can be linked to LLM Neuroplatform - FinalSpark , finalspark.com/neuroplatform/ can be linked to chatGPT ? What to do with this ? Avatar Pilots ? Studying the case of...

28 May 2024 3,302 0 View

Tumor Organoid Growth ?

Hi everyone, I am culturing colorectal cancer organoids (Control and Experimental) and I observe a faster growing (number and size of organoids) in experimental condition. Now the question is,...

27 May 2024 9,258 0 View

Troubleshooting western blot for 200 kD Protein. Can anyone help?

The truth is that I have had many, many problems to be able to see a specific band of this protein. My problems have ranged from bad transfers, to unknowns that I have not been able to answer. The...

26 April 2024 8,595 4 View

Using a preform analyzer to measure the refractive index profile of other samples than silica preforms?

Hello I have to work with a preform analyzer model 2650 - Photon Kinetics, but I have no preforms yet to start the calibration and the tests. Is it possible use other kind of sample with a well...

25 April 2024 564 1 View

I'm doing high molecular weight western blot (above 200) but I'm doing low weight endogenous blot (37), how can I detect both?

Although I can detect the protein (above 200), leaving it for longer in the transfer (plus 2h), I think I am losing my endogenous protein, as its detection is not getting good. How can I solve it?

07 April 2024 2,338 3 View

Why does my protein refolded to beta sheet during thermal denaturation analysis?

Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...

06 August 2024 1,989 3 View

Transfection in HEK293T cells?

Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...

31 July 2024 9,892 4 View

How to retain the a GFP tagged gene expression in stable cell line?

Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....

29 July 2024 5,007 0 View

What is the relationship between protein structure and N or C terminal tagging choosing?

I want to do 2,3-butanediol dehydrogenase(BDH) enzyme purification to confirm its activity for 2,3-butanediol. Before that, I need to confirm which N or C terminal tagging is better for enzyme...

28 July 2024 366 3 View

Why cannot i find my protein on cell surface after antibiotic selection of expressing plasmid?

I cannot confirm cell surface protein expression by flow cytometry even after transfection and antibiotic selection of cells. Does it take long for proteins to express on the cell surface? the...

28 July 2024 3,178 3 View

EDC carbodiimide and NHS easter 's half life among different pH?

It has been long known that EDC carbodiimide and NHS easter's half life agains hydrolysis are highly related to pH. However, very surprisingly, not yet found a paper giving a table/chart of their...

25 July 2024 8,738 0 View

Does post-translational protein modification cause devisions on observed pI verses calculated pI?

In running two-dimensional gel electrophoresis on bacterial protein, some spots that appear to match a protein sequence have a significantly more acidic isoelectric point than the calculated pI....

24 July 2024 8,076 3 View

What is the function of fusion tag GAMGSGIQRPTST?

I found that several expression constructs of published protein structures containing an N-teiminal fusion tag GAMGSGIQRPTST. What's the founction of this fusion tag?

23 July 2024 1,353 4 View

The best source for amplification of ADAM17 prodomain?

hi every one I am making vector construction (for fusion proteins) and in this moment I wanna to amplification of ADAM17 prodomain with PCR. to yet, I couldn't amplified the ADAM17 prodomain with...

21 July 2024 8,660 1 View

How to estimate a protien's size using Chimera or PyMol? how correct this estimated can be?

I need to know the approximate size of my target protein for Negative-stain EM. I have an alpha-fold predicted structure of the protein. I do not see any turtorial on internet for estimating the...

21 July 2024 5,732 3 View