Cell infection with lentivirus: GFP+ after soring by flow but no protein expression?

I use a plasmid to overexpress 1 protein in 293T and murine BMSC. But I could never succeed. I produced the lentivirus by transfecting the plasmids in HEK293FT cells, together with another 3for...

24 December 2020 6,389 3 View

I keep getting an error for GEOPIV on Matlab?

When doing the geoPIV_RG_run, i keep encountering this error. Error using dataread Trouble reading floating point number from file (row 1, field 2) ==> Dynamic Load Test Free Vibration Error...

20 October 2020 10,004 2 View

Is lentiviral production of antibodies in 293T viable?

Hi, I recently started using lentiviral systems to produce proteins in mammalian cells. Knowing the performance of this impressive system in producing high titers of proteins, I am wondering if...

05 August 2020 5,144 2 View

What solutions do you suggest for molecular field visualization/analysis?

Hello, I wonder what options exist these days for visualizing "molecular field" type data. That is, a 3D scalar field represented by translucent volume rendering or isosurfaces, and overlaid on a...

12 June 2020 861 2 View

How to choose collective variables for ligand binding to protein?

Hello! What is the recommended way to choose collective variables / reaction coordinates to study ligand binding to a protein (e.g., for PMF free energy profiling)? I often see the advice like "Do...

10 March 2020 1,878 4 View

No title

02 November 2019 8,943 3 View

Why an SD of means difference between two groups (e.g. treatment vs. placebo) is equal to a particular SD in each group?

Very frequently in papers, devoted to parallel clinical trials, I face a situation, when a calculated SD for an effect in each group is approximately equal to an SD of effect difference between...

07 July 2019 9,217 4 View

What aproach we must use to calculate force in electrostatic problems: potential or electric field intensity?

Well known problem of finding potential for metal sphere and point charge, solving with "mirror image" trick give you 2 different answers for the force, acting on point charge. One when we use...

14 December 2016 9,365 5 View

Does choline hydroxide (46% aqueous) ionic liquid still absorb water from surroundings?

I will be using this ionic liquid as an electrolyte for my battery experiments.

01 August 2016 2,559 1 View

Hall Sensor and EMC?

What would be your brief recommendations for EMC for Hall sensors? I mean; how to increase robustness of the Hall sensors circuits? Any links? Thank you, Eugene

27 April 2016 8,388 9 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

How to handle studies which report perfect effect sizes in a meta-analysis using Pearson's r?

I'm in the process of updating a meta-analysis and in one of the new studies, effect sizes are reported as Tau-U. Two of the effect sizes are reported as Tau-U = 1.00. I am using Andy Field's...

24 February 2021 2,265 3 View

How to read a flow cytometry graph detailing cell cycle?

Hello everyone, When determining cells in different stages of the cell cycle, cells are stain for Brdu and Propidium Iodide. I understand how PI can be used to discriminate between G1 and S/G2...

18 February 2021 3,920 3 View

How to report Fishers Exact test from SPSS in a report properly?

I am wondering how do I write the data I have collected from my fishers exact test into words so that it backs up my work? the exact sig.(2-sided) was 0.033, meaning it is significant because its...

17 February 2021 3,441 7 View

Aspen Plus Error: Unknown or undefined bead number?

Hello, in my Aspen Plus Simulation (see attachment) there are two heat exchangers. One HEX1 (oil+gas) ist fed by hot gas which ist coming out of a burner. The other HEX2 (gas+gas) shall work as a...

14 February 2021 8,096 2 View

GST assay in microplate?

I'd like to know if anyone can provide me with a protocol for traditional GST enzymatic assay (with GSH and CDNB as substrates) in a 96 well microplate, for cell line and/or C.elegans...

11 February 2021 1,328 3 View

Does anyone have a stack of images of action potentials or abnormal brain scans?

I need a stack of black and white .jpeg/.tiff/.jpg/.png images across time of either action potentials, neurons firing, or brain scans (comparing disease and normal brain, disease progression,...

09 February 2021 2,648 3 View

How to test the equality of two proportions with multiple samplings?

02 February 2021 9,574 5 View

Western blot transfer problem - what could cause it?

Hi everyone, We are doing western blot with yeast protein extracts. We are applying a wet transfer method using Tris-Gly as a transfer buffer (with 10% methanol) and running it with constant...

25 January 2021 4,228 6 View

Is Juniper Publisher ( Journal of Gynecology and Women's Health) a good journal to publish my paper?

Dear colleagues, Do you have any knowledge about Juniper Publishers? Have any of you published in the Journal of Gynecology and Women's Health ( ISSN: 2474-7602, Impact Factor: 0.621 (2017-18))? I...

21 January 2021 9,362 4 View