Hi dear Matthew

Sometimes you find questions similar to your question. So please follow the link below

https://www.researchgate.net/post/How_to_use_DNeasy_with_snake_scales

Hi Mathew,

It's important to distinguish between DNA yield and DNA concentration. If you wanted to increase your DNA concentration, the best way would be to re-precipitate your sample (via your protocol) and then resuspend in a smaller volume of your buffer of choice.

So far as DNA yield there is the obvious choice - to increase the amount of tissue that you're extracting DNA from. Another option I've seen folks do is to warm their elution buffer (around 40C) and/or let the DNA column sit for 5 minutes after adding the elution buffer. I would also try doing the elution step twice to get a little more DNA out of the column.

Yet another option is to divide your sample into two different DNA extractions and combine them at the very last step (after elution, move contents of tube 1 to tube 2). Total DNA yield is typically higher in this case.

Another factor to consider is the DNA kb - something large (>5kb) may not be compatible with the kit you are using.

Good luck!

Dear Matthew,

Sometimes low concentration is not really a bad thing!

It all depends on the use of the DNA. If your DNA is having low concentration but good purity, you can still yield good results after downstream process such as PCR and NGS using specific primers. The information obtained from the PCR or NGS service can later be used in molecular phylogenetic analysis.

However, if the samples is having both low purity and low concentration. You can consider to use commercialize kit from some company (e.g. Qiagen, Thermo Scientific etc.). You can also have a read on the attached protocol.

All the best!

Hi dear Matthew

Sometimes you find questions similar to your question. So please follow the link below

https://www.researchgate.net/post/How_to_use_DNeasy_with_snake_scales

Thank you all! I appreciate the helpful feedback