Mukaram Bhat

9 Questions 5 Answers 0 Followers

Questions related from Mukaram Bhat

How to Achieve Specific Knockdown of a Gene Variant That Shares High Sequence Similarity with Other Variants?

I am working on a gene that has 14 variants, and I am specifically interested in knocking down one particular variant. After sequence alignment, I discovered that the variant I want to target has...

21 January 2025 9,591 0 View

How can I identify the promoter sequence of a specific gene and determine which transcription factors bind to it?

I am currently studying a particular gene and am interested in understanding its regulation. To proceed, I need to identify the promoter sequence of this gene and investigate which transcription...

11 January 2025 5,870 2 View

How to filter out apoptotic genes specifically from a set of genes?

Is there a tool that can identify both upregulated and downregulated genes from RNA-seq data that are specifically involved in the apoptosis pathway? I am interested in filtering for genes linked...

13 October 2024 5,802 1 View

Seeking Guidance on Variant Analysis in Differentially Regulated Genes using RNA-Seq Data?

I am analyzing RNA-Seq data, specifically focusing on the identification of variants within differentially regulated genes across treated and untreated samples. My primary analysis platform for...

02 January 2024 4,757 0 View

Any suggestions on obtaining epitope information for a specific splice variant of gene X from Cell Signaling or Abcam antibodies?

I am currently in need of an antibody targeting gene X, specifically focusing on a distinct splice variant where the major variance lies within the N-terminal region. Unfortunately, suppliers like...

27 December 2023 8,495 2 View

How to find the information about a particular transcript variant using a genome browser?

What tools or methodologies are available to comprehensively analyze RNA expression profiles across various human tissues, specifically to discern and compare transcript variants of a gene

20 December 2023 7,196 7 View

Conversion of RNA Seq data from fastq.gz to .bw?

I possess RNA-Seq data in fastq.gz format and I'm seeking guidance on transforming it into a suitable BigWig (.bw) format. What steps or tools are recommended to convert FASTQ files to BigWig...

17 November 2023 6,936 2 View

What can be the other possibilities of not getting any colonies after transformation if the efficiency of competent cells is low?

I am doing Pcr cloning trying to clone a 3000 bp pcr product into a 9000 bp pcdh_bec1 plasmid .digestion was done using Xba1 and Not1 ,after gel extraction loaded 2ul on the gel and observed faint...

28 September 2023 3,137 2 View

Do we need to add dNTPs in 3'overhang chewing by klenow fragment? if yes,Why?

while doing klenow reaction for blunt end cloning,i am not getting why it is necessary to add dNTPS to chew 3' overhangs

24 March 2023 3,834 1 View