I want to avoid chaotropic reagents as they are supposedly toxic and as I am developing a point of care diagnostic protocol for ZIKA to be used on the field (not under normal laboratory conditions), I would prefer a non-toxic reagent.
Are you looking to isolate ZIKA RNA from serum?? Perhaps try using Kosmotropic salts which work by increasing water surface tension rather than disrupting water structure to encourage hydrophobic interaction hence protein precipitation.
Here's a paper that decribes isolation of RNA from E.coli using this method. How suitable this might be for Viral RNA isolation I've no idea......good luck
Analytical Biochemistry
Volume 381, Issue 1, 1 October 2008, Pages 160-162
Isolation of total RNA from Escherichia coli using kosmotropic Hofmeister salts
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