Kindly some one help me to calculate the Peff value from the small intestinal perfusion study. We are collecting samples for the regular interval of time and analyzing the concentration (Cout). So we will get "n" number of values.
With the equation we can calculate the Peff value. Peff=-Q*ln(Cout/Cin)/(2*pi*rL).
Here which Cout value I should take. Is it the average or summation of all the Cout values. Please help.
Thanks in advance
Dr. Razzaq Muhammed,
Thanks for the immediate reply with the relevant articles. Those experiments were carried out on the humans, I'm trying just with the animals only.
Many research were carried out with the In-situ small intestinal study in animals and many research articles also published. Authors providing the equation and giving the value. No where they mentioned about it.
Thank you so much for giving the valuable reply. Actually I'm going to find the effective permeability of a emulsion. In most of the research articles, Peff was calculated for the drug. So they perfused the drug in the intestine and checked the difference in concentration.
My query is why the researchers are not using any digestive enzyme? Why the gastriointestinal digestion was not accounted during the perfusion study? Some researchers used Pancreatin in the buffer. Is that enough for digestion. As I'm using the emulsion, with protein as a surfactant, I need to digest the material before entering the intestine. Can I follow the Pancreatin digestion..
Kindly suggest a method..
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