I am using an Epsion machine with a Ganzfield dome stimulator, with subcutaneus needle electrodes (nose, tail, head). Anesthesia with ketamine/xilazine (injected i.p. 13 minutes after the exam)
I am putting the head needle electrode in exactly the same place every time, but during flash VEPs in the same healthy mice I am frequently finding latency differences of 10 ms. (i.e. 65 ms first day, 75 ms second day, 68 the 3rd, etc).
Any suggestion?
Thanks in advance.
Hi Christian,
usually VEP amplitudes are more sensitive to experimental condition (e.g electrode location and depth), whereas latencies measurements are less affected.
Variables that I've found affect latency are:
- mouse body temperature during recordings;
- hour of the day of VEP recordings.
Try to record VEP at the same hour of the day and mouse body temperature (with circulating water heating pad t 37° C).
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