We've been using the MC38 s.c. tumor model and continue to have issues with tumor ulceration. We have tried injecting in different locations, varying the cell number, varying the resuspension buffer (PBS, RPMI, matrigel), using different cell lots, etc. The cells are myco-free and have been MAP tested. This is not a tumor size issue as the ulcers appear in small tumors (~200 mm3).
Has anyone had success in reducing the ulceration frequency?
Hi John,
Try intra-rectal administration of rebamipide.
(Actions: radical scavenging, mucus-stimulating, anti-inflammatory)
Best Reg,
S
Hi John,
Please check this recent publication using Salmonella for cancer treatment
Two-step enhanced cancer immunotherapy with engineered Salmonella typhimurium secreting heterologous flagellin. Sci Transl Med. 2017 Feb 8;9(376).
http://stm.sciencemag.org/content/9/376/eaak9537.full
Best,
Ashleigh,
Thanks for the comments. I, too, am amazed by publications with this model. How can they 'realistically' and 'accurately' measure such tumors with large holes in them?
Hi John,
Did you ever find a solution for the ulceration issue? We're not having much luck with this model either.
Hi Sarah,
We haven't. I've seen an ad for a company that actually sells the MC38 cells now. I'm not sure if they would be better/worse than those exchanged between labs. I'm also hoping that ATCC or other analytical labs would finally establish a STR DNA profile for the cells. Then, one can see if you actually have authentic cells. It may not help with ulceration though. I guess the only way to handle the ulceration is to include additional mice/group and eliminate those with ulcers.
Good luck.
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