Vu Hong Nguyen

2 Questions 16 Answers 0 Followers

Questions related from Vu Hong Nguyen

How do I select a donor vector for knock-in a gene using CRISPR-Cas9?

I have a plan to knock-in a gene with about 2kb in size into a cancer cell's chromosome. I suppose to make a DSB (double strand break) using CRISPR-Cas9 system then a gene (2kb) will be inserted...

08 July 2016 2,202 8 View

Solution in IF staining?

I am trying to stain CD68 and CD11b on tumor tissue (mouse). I have collected mouse tumor tissue, fixed it in 4% Paraformaldehyde, dipped it in 30% sucrose and cryo in OCT -80oC. The tissues...

30 August 2013 4,315 10 View