Why am I not getting colonies using the Agilent QuikChange II kit?

Hello everyone, I hope you are very well. I am performing site-directed mutations with the QuikChange II kit. My first ones have a length of 50bp and a Tm of between 75 and 81 approximately. I...

09 November 2020 242 2 View

Exist some reliable website with information about seric calcium and phosphorus in Chelonoidis chilensis?

Someone will have papers, books or magazine articles about it. It's for my thesis. Thanks

03 June 2019 9,730 2 View

Which one is the best protocol or extraction step for RNA extraction in 3T3-L1 cell line?

I used the standard protocol for this, but the lipids still there. they should be in the top but it doesn't appear, so when I use the chloroform the lipids are in the top of the organic phase

11 December 2016 2,775 10 View

Which (python) workflow could I use to manage meteorological data?

07 June 2016 6,150 5 View

Differences between pGEMT and pGEMT Easy Vector??

Hi. I need to know what are the differences between both pGEMT and pGEMT Easy Vector. I need to cloning a PCR product of ~1300pb. Thanks!

17 August 2011 9,936 18 View

How to add missing citation?

Dear colleagues, I have found that many authors have a concern regarding their missing citation in RG because often citation in RG is lower than google scholars. I also missed some citation...

26 February 2021 928 6 View

Can we take 96 well plate readings in three different plate readers ( different companies) for 12 hr,24hr and 48 hrs? With same 540nm wave length ?

My experiment which require 12hr,24hr and 48 hrs readings at 540 nm wavelength with referenc 560 nm wave length after mtt assay and dmso treatment . Can i take readings of 12 hr ,24 hr and 48 hrs...

09 January 2021 5,989 2 View

Which style is more popular; MLA, Chicago, or APA Style in writing an excellent paper? Why?

Writing a good paper or article considered to be a great effort in order to reach incredible results. Hence, which style do you think is more popular and better to be used? Is it MLA Style,...

26 December 2020 173 20 View

Which is the best referencing tools ,website...?

We get difficult in referencing, and not getting any reliable site

22 December 2020 9,386 4 View

Experimental Data Set of Sluice Gates Under Free Flow Regime?

Could anybody share a dataset for the sluice gates operating under the free-flow regime? I need the upstream and downstream depths as well as the opening of the sluice gate at least. Any help...

17 December 2020 4,405 1 View

Is it acceptable to publish data that were digitized from a previously published plot/figure (referencing the original figure of course)?

There are many ways to extract original source data from figures and plots in publications. If one were to do that and reanalyze the data in the same or a different way, is that acceptable in a...

28 October 2020 3,822 2 View

What is this citation style called?

Hello everyone, I am preparing a manuscript for a journal which indicates in the authors guidelines that the citation and referencing style should look like the following: Citation in the text...

22 September 2020 6,897 6 View

When and where should we use a reference(s) in writing a research paper?

Of course we use references when we quote someone else statements or findings, however, sometimes they are bombard to look a research paper heavy. Can any explain when do we need to use a...

10 August 2020 3,123 6 View

How and where do I get a reflective essay reviewed and published?

I spent the past few months interning as an independent researcher at the Field Museum in Chicago. Here, I spent my time observing the design procedure that leads up to the creation of a...

23 May 2020 770 10 View

Is there a referenced dataset for extractive/abstractive single document Summarization ?

is there a referenced dataset for extractive/abstractive single document Summarization?

07 May 2020 4,087 9 View