Why am I not getting colonies using the Agilent QuikChange II kit?

Hello everyone, I hope you are very well. I am performing site-directed mutations with the QuikChange II kit. My first ones have a length of 50bp and a Tm of between 75 and 81 approximately. I...

09 November 2020 242 2 View

Exist some reliable website with information about seric calcium and phosphorus in Chelonoidis chilensis?

Someone will have papers, books or magazine articles about it. It's for my thesis. Thanks

03 June 2019 9,730 2 View

Which one is the best protocol or extraction step for RNA extraction in 3T3-L1 cell line?

I used the standard protocol for this, but the lipids still there. they should be in the top but it doesn't appear, so when I use the chloroform the lipids are in the top of the organic phase

11 December 2016 2,775 10 View

Which (python) workflow could I use to manage meteorological data?

07 June 2016 6,150 5 View

Differences between pGEMT and pGEMT Easy Vector??

Hi. I need to know what are the differences between both pGEMT and pGEMT Easy Vector. I need to cloning a PCR product of ~1300pb. Thanks!

17 August 2011 9,936 18 View

How do I test for gender differences in Spearman rho correlations?

I want to explore if there is a gender difference in a correlation between two ordinal items. I have calculated spearman rho within gender for the items, but I now need to test if the correlation...

09 October 2020 970 13 View

What are the effects of gender of performer on animal?

I remember a paper studied the effects of gender of handler on animal (mouse?) showing that male handler has more negative effects on animal (for example, causing animal anxious) compared to...

05 August 2020 4,131 1 View

Are woman who have an hysterectomy more likely to experience mental illness following surgery?

The evidence is mixed and variable over the decades. More recently, evidence suggests mental illness can be associated with having an hysterectomy. Case Study A lady, aged 54 had an hysterectomy...

10 April 2020 7,324 4 View

Are fMRI or DTS scans being used for detecting learning challenges among students?

There are several cognitive challenges even a normal person can face (ref. Barbara Arrowsmith, "the woman who changed her brain"). How frequently are brain scans being used in common domains...

16 February 2020 2,078 3 View

LIMB Jones Dress Short XS check: #400021578?

This is not a question but a 'cut and paste' that the computer I'm using right now decided was a question. So going with the 'flow' so to speak I will try to turn this 'cut and paste' into a...

28 December 2019 6,962 1 View

How would you explain a case with unusually strong expression of p16ink4a in human endometrium?

The endometrial biopsy was taken in the mid-lutheal phase of the menstrual cycle from a woman with recurrent implantation failure (RIF). Extremely high percentage of stromal and luminal epithelial...

11 June 2019 2,583 1 View

Evidence of peaceful coexistence in Islam with the infidels and other sections of infidels What are they?

I am writing a paper on peaceful coexistence with the infidels in Islamic law

20 May 2019 8,103 7 View

Woman in computer science?

opinion

02 March 2019 6,588 13 View

Happy Woman's Day for you?

Happy Woman's Day for you where you're. https://www.youtube.com/watch?v=aJOTlE1K90k

02 March 2019 7,042 25 View

In your opinion, as a woman .............From your point of view as a man .................. Does cosmetic surgery change your personality positively?

In your opinion, as a woman....... From your point of view as a man .......... Does cosmetic surgery change your personality positively?

31 December 2018 9,117 21 View