Indirect fluorescent antibody (IFA) assays to detect localisation of a protein in serum/plasma?

On finding the a protein of interest in human serum and plasma samples, I'm now interested to find out regarding the localisation of the protein; is it free floating, membrane bound with vesicles,...

02 March 2014 993 0 View

4kb mRNA transcript for first strand synthesis; use random hexamers or oligo dT?

I am looking at doing RT-PCR for an mRNA target of about 4kb long, and primers will be targeted close to the 5' end due to splice variants at the 5' end that I'm interested in looking at. I'd be...

01 February 2014 8,541 5 View

Singapore: Climate departure, and La Nina? Or complicated by climate change arising from global warming?

Something outside my expertise, but I would like to ask in view of the recent Nature paper that says Singapore will likely see climate departure in 2028 (Mora et al., 2013) if nothing was done in...

09 October 2013 5,099 2 View

GFP tagging of the same protein, but produced in different locations?

A rather far fetched thought, but is it possible in animal models to tag the same protein with different fluorescence proteins genetically to represent production in different parts of the body...

09 October 2013 8,114 2 View

Are there any robust human tests for hippocampal-dependent learning and memory?

Any tests that allow us to assess the above without MRI use even?

06 July 2013 6,561 1 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

No title

02 March 2021 3,060 3 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View