Jun Jie Lee

6 Questions 8 Answers 0 Followers

Questions related from Jun Jie Lee

Indirect fluorescent antibody (IFA) assays to detect localisation of a protein in serum/plasma?

On finding the a protein of interest in human serum and plasma samples, I'm now interested to find out regarding the localisation of the protein; is it free floating, membrane bound with vesicles,...

03 March 2014 1,023 0 View

4kb mRNA transcript for first strand synthesis; use random hexamers or oligo dT?

I am looking at doing RT-PCR for an mRNA target of about 4kb long, and primers will be targeted close to the 5' end due to splice variants at the 5' end that I'm interested in looking at. I'd be...

02 February 2014 8,575 5 View

Any recommended protocols for receptor density quantification?

Does anyone have good protocols to share for cell surface receptor quantification, within blood cells in particular?

11 November 2013 2,540 4 View

Singapore: Climate departure, and La Nina? Or complicated by climate change arising from global warming?

Something outside my expertise, but I would like to ask in view of the recent Nature paper that says Singapore will likely see climate departure in 2028 (Mora et al., 2013) if nothing was done in...

10 October 2013 5,131 2 View

GFP tagging of the same protein, but produced in different locations?

A rather far fetched thought, but is it possible in animal models to tag the same protein with different fluorescence proteins genetically to represent production in different parts of the body...

10 October 2013 8,145 2 View

Are there any robust human tests for hippocampal-dependent learning and memory?

Any tests that allow us to assess the above without MRI use even?

07 July 2013 6,595 1 View