The Joule-Thomson effect?

Hello I recently am suffering calculation of temperature change resulted from the Joule-Thomson effect when propylene gas at 35 oC at 2 bar passes through a membrane to vacuum pressure (~ 0 bar)...

23 December 2020 4,091 2 View

When available, are you planning to receive SARS-CoV-2/COVID-19 immunization? What do you most fear about it?

If possible, let us know about the which vaccine would be available in your geographic location.

06 December 2020 3,527 23 View

How to invite call centers to participate in your study?

I'm conducting a job satisfaction study to predict telephone agents' turnover. I'm looking to increase participants for my study. How should I approach the directors of call centers to request...

20 September 2020 7,099 8 View

What is the correct statistical test for this data?

I have data on a bacteria (Group B Strep) that colonizes women but it can also cause infection (it's a sneaky little bug). I want to see if there is a difference between pregnant and non-pregnant...

13 August 2020 5,805 10 View

How to avoid incomplete responses?

I'm conducting an anonymous quantitative survey on SurveyMonkey and some participants left the majority of the questions unanswered. How can we encourage participants to completely answer all...

22 June 2020 8,202 4 View

How to calculate specific capacity in C/g from a CV curve?

I am newbie to battery materials. As I understand, specific capacity of a battery-type material can be expressed in term of C/g or mAh/g and can be calculated from the cyclic voltammetry (CV) or...

06 May 2020 4,202 6 View

Have you applied 3D Laser scanning and Building Information Modeling for facility management during operation phase?

14 March 2020 7,806 3 View

I am looking for a good antibody to use as loading control for rabbit's proteins in Western blot. Any recommendations?

I tested two primary antibodies anti-beta-actin so far, and I am getting very inconsistent results accross my Western blots, sometimes I don't even get any detection, and sometimes I get...

02 December 2019 493 2 View

EDS Mapping how to get better results?

Hi everyone, hope you're all okay. So I have some questions/doubts about eds mapping. Just some notes first: I always use the correct accelerating voltage to excite the elements I need, always use...

04 June 2019 2,854 11 View

Histogram on a Scanning Electron Microscope, how to interpret?

Hey folks, hope you're all okay. I have one doubt, I always used to check the videoscope to help improve the bright and contrast in the microscope, but I used to do this in a FEI (Thermofisher)...

17 May 2019 4,055 4 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

Anyone know of a good progesterone antibody?

I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.

28 February 2021 5,929 2 View

Benefits of the 24 welled plate?

We have always used the 96 welled plate, the 48 welled plate but apparently there is much evidence on the 24 welled plate and above all its benefits.It states it is the same you would use on ELISA...

28 February 2021 1,149 1 View

What are the pros and cons for mice versus rats?

Hi Everyone, I am designing a pilot study using rodents to assess the effects of simulated space environments (radiation + altered gravity) on microbiome and immune system. I have limited...

24 February 2021 1,825 3 View

Standard curve shifting over time. How do I stabilise the ELISA?

24 February 2021 1,365 3 View

IF troubleshooting: looking at an intracellular protein that localizes to the cell's and how to reduce increase in signal due to cell ruffles?

I am currently trying to stain for PI3KBeta in MDA-MB-231 cells. My current issue is that 1) beta stains all over the cell and 2) there is an increase in signal at the edge of the cell and this...

24 February 2021 8,582 1 View