May I know who successfully knock-in target gene in mice? Using what methods?

Would appreciate if you can share how you done it. Thank you.

07 February 2018 2,091 1 View

Is the oxygen level monitored in the incubator important?

In our lab. we have 2 versions of cell culture incubator, one is an old fashion without oxygen controlled, the another is new designed with tri gases; nitrogen, oxygen and CO2. Will the new...

21 May 2015 9,550 3 View

Where might one buy particulate matter (PM) 2.5 um?

03 November 2014 3,893 9 View

What command do I use when I want to calculate the surface tension on GROMACS?

I have a system with two interfaces, and I would like to calculate the surface tension.

08 October 2014 6,558 1 View

Has anyone used Cayman's Catalase Assay Kit? What is the protein concentration used for cell lysate samples?

What is the optimal protein concentration of cell lysate used for this assay kit?

08 September 2014 8,749 3 View

Has anyone used Cayman's Catalase Assay Kit? What is the protein concentration used for cell lysate samples?

What is the optimal protein concentration of cell lysate used for this assay kit?

07 September 2014 2,943 2 View

Is there any work in the literature comparing the surface tension of spin-cast thin film of PMMA with different tacticities?

If I prepare thin films of atactic and isotactic PMMA by spin-coating method and measure contact angles of deionized water on these films under room condition, are the contact angles of these two...

23 July 2014 6,423 1 View

Does anyone have experience using a 3D cell culture novel screening method for anticancer agents?

3D cell culture in liquid form. This is quite new to me. Usually, I always heard about 3D collagen gel model for cell profiration or migration. In this new case they (Nissan Chemical Industries)...

24 July 2013 992 3 View

NF-kB and cytokines?

If NF-kB activation could not be detected, is it possible that the cytokines will be produced? I'm working with macrophage cells. Nitric oxide production can be detected after treating the cell...

04 May 2013 5,987 5 View

What is a good performance for Cytocentrifuge?

We are looking for a brand new cytocentrifuge which will be used for cell sedimentation and fixation. Since this is very new to me, are there any comments or criteria should I have to...

24 April 2013 4,176 3 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

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02 March 2021 3,060 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View