To enrich Artemia, we can feed with vitamins or calcium or by adding an emulsion of phospholipids rich in DHA to newly hatched Artemia. The Artemia eat the emulsion. The Artemia are then fed to the fish or can then be kept refrigerated for up to 3 days. Feed the artemia minimum of 12 hours before feeding them to fishes or other organism...
Among the zooplankton, brine shrimp (Artemia spp.) and rotifers (Brachionus plicatilis) have been used extensively as live food for rearing marine and freshwater shrimp/fish larvae. The use of Artemia nauplii is well established due to its many advantages: year-round availability as on- the shelf cysts; good nutritional value for some shrimp/fish and relatively easy improvement through simple enrichment techniques. Nutritional deficiencies have been another concern other live food. The essential fatty acids for shrimp are broadly recognized to comprise PUFA with carbon chain lengths of C18 and HUFA with C20 and C22, of both the n-3 and n-6 series. These fatty acids cannot be synthesized by the shrimp, though the C18 PUFA can be converted by some species to longer-chain, more highly unsaturated fatty acids of the same series. On the other hand, ascorbic acid is also an important micronutrient in the shrimp diet. It is needed for stress, good survival, and to boost the immune system. Hence, these fatty acids and vitamin C should be provided in the diet to meet the shrimp larvae requirements. In this line, there have been extensively studied research findings available check it.
@niteshkumaryadav: Many thanks. Usually in artemia hatching there is more possible for vibrio growth. How to avoid vibrio load in 12 hrs enrichment process?
The growth of vibrio load is already suppressed if you wash artemia cysts in light bleached water which is a common practice in the prawn hatchery to innoculate artemia cysts.