What are the easy methods or techniques to synthesize green nano particles?

because many technical steps are there to get a particular plant nano particle and its expensive too

14 January 2021 1,556 4 View

For a PCR the volume of each component is standard ?

for example the total volume is 50microliters how can we know that what will be the amount of template , buffer, enzyme, primers (forward and reverse), dNTPs, MgCl2 and water?

02 January 2021 3,352 6 View

How can we know about the final concentration of each component to be used in the master mix for the PCR ?

Final concentration for each component in master mix depends on what or how it will be known that in this master mix the concentration required is __ microliters ?

02 January 2021 5,764 2 View

How can I separate features and the background to measure pixel intensity?

For image processing, I am using ImageJ. I need to separate the features from the background and calculate pixel intensity of features and background. Can anybody help me in this? Thanks

09 December 2020 7,472 9 View

Which are the appropriate metrics can be used to calculate overall Precision, Recall and F-measure in different data samples? and when can use F1?

If we have three different domain of data (e.g. security, AI and sport) and we did 3 different case study or experiments (1 for each domain) and we estimate the Precision, Recall and F-measure for...

07 December 2020 9,833 3 View

How can stem cells repair damaged cells?

How can stem cells repair damaged cells? Could anyone provide papers show the therapeutic potential of stem cells in vitro (on the disease model)?

29 November 2020 9,534 6 View

How can to calculate the Computational complexity/cost (espatially, number of operations and space/memory cost) for an algorithm?

As we know, computational complexity of an algorithm is the amount of resources (time and memory) required to run it. If I have algorithm that represents mathematical equations , how can estimate...

25 November 2020 6,950 7 View

Which is the best scale available to measure conflict management styles?

I have come across multiple instruments for conflict. Now I'm confused as to which one to use. Expert opinions are welcome.

22 November 2020 3,651 5 View

Chi square test for the over time tested variable ?

16 November 2020 8,291 4 View

Help me find the transfer function of the following non linear coupled ODE system ?

I need to find the transfer function for the system in MATLAB. I do know the ss2tf command but its not that simple. Please guide.

08 November 2020 4,414 1 View

What are the countries most affected by extreme rainfall as a result of climate change?

Increased heat, drought and insect outbreaks, all associated to climate change, have increased wildfires. Decreasing water supplies, declined agricultural yields, health impacts in cities owing to...

01 March 2021 7,168 9 View

How to accurately quantify chitin?

Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...

01 March 2021 1,843 2 View

Which microtomy technique do you suggest for thin and delicate root sectioning ?And, how to preserve and process the roots before sectioning?

We have thin roots of from 1 year old different species of plants. We are looking into anatomical details of the roots. What's the best method to preserve and process the roots once they are...

23 February 2021 1,898 1 View

Please help in identification of this insect?

This insect is minute in size around 1 mm and not clearly visible with naked eye.

22 February 2021 5,907 11 View

Purification of proteins from baculovirus-infected insect cells?

18 February 2021 6,665 6 View

How to successfully revive cells stored at -80 degree C for a year?

I am working on C6 cells along with other neuronal cell lines. We have preserved the cells in 90%FBS and 10% DMSO solution. We are reviving the cells after a year and cells are either not binding...

18 February 2021 4,499 1 View

Extraction of DNA from insect samples conserved in silica gel?

Hi everybody, can anybody suggest how to extract DNA from insect samples conserved in silica gel (in my case, mosquitoes)? Does anybody know a commericial kit which does that or a particular...

10 February 2021 3,392 4 View

How do I prepare and store insect samples for JH measurements?

I need to preserve termite samples for future quantification of juvenile hormone in their bodies. Or extract the Juvenile Hormone and keep that samples stored for future quantification. I may need...

09 February 2021 1,995 1 View

Working with insect primary cells in cell medium and in saline solution?

I work with insect primary cells, which I let grow in TC100 insect medium. For fluorescence measurements I have to discard medium (because of its fluorescence properties) and give a saline...

28 January 2021 3,867 1 View

How to extract a single stranded DNA from a double stranded DNA sample isolated from stem borer(rice affecting insect) ?

How to extract a single-stranded DNA from a double-stranded DNA sample isolated from stem borer(rice affecting insect)? As I need to amplify ss Dna using a COX-1 marker having primer sequence as...

22 January 2021 3,424 3 View