Give me Technical Guide on maintain an appropriate balance and avoid excessive supplementation while downing Salinity in L.Vannamei's PL stage?

@all Lowering the salinity level in a shrimp larval rearing tank requires careful management to minimize stress on the shrimp post-larvae (PLs). Here is a technical guide to decreasing salinity while reducing stress:

Gradual salinity reduction: It is crucial to lower the salinity gradually to minimize stress on the PLs. Sudden changes in salinity can be detrimental to their health. Aim to reduce the salinity by a maximum of 3-5 ppt per day, depending on the tolerance of your specific shrimp species.

Prepare a freshwater source: Ensure you have a reliable source of freshwater with consistent quality and appropriate temperature. The freshwater should be free from contaminants and should have parameters suitable for the shrimp larvae.

Dilution calculation: To determine the amount of freshwater required for each salinity reduction step, you need to calculate the volume of water to be replaced. The formula for calculating the volume of freshwater needed is:Freshwater volume (in liters) = Total tank volume (in liters) x (Initial salinity - Target salinity) / Initial salinityFor example, if your total tank volume is 1000 liters, and you want to reduce salinity from 25 ppt to 0 ppt:Freshwater volume = 1000 x (25 - 0) / 25 = 1000 litersThis calculation indicates that you would need to replace 1000 liters of tank water with freshwater.

Calcium and magnesium supplementation: Adding calcium carbonate and magnesium chloride can help compensate for the mineral loss during salinity reduction. These minerals are important for shrimp health and osmoregulation. However, there is no fixed formula for determining the exact dosage for every salinity reduction step. It is recommended to consult with a shrimp aquaculture expert or a veterinarian familiar with shrimp nutrition to determine the appropriate dosage based on your specific requirements.

Monitor water parameters: Regularly monitor the water parameters such as temperature, pH, and dissolved oxygen during the salinity reduction process. Sudden changes in these parameters can also stress the PLs. Make adjustments as necessary to maintain stable and optimal water conditions.

Observe shrimp behavior: Carefully observe the PLs for signs of stress, such as increased mortality, erratic swimming, or reduced feeding activity. If you notice significant stress indicators, consider slowing down the salinity reduction process or seek expert advice.

Remember, each shrimp species and even individual populations may have varying tolerances to salinity changes. It is crucial to closely monitor the behavior and health of the PLs throughout the process and make adjustments accordingly to ensure their well-being.

Ahmad Al Khraisat

@all Decreasing the salinity level in a larval rearing tank can be a delicate process, and it's important to minimize stress on the L. Vannamei post-larvae (PLs) during this transition. Here's a technical guide to help you decrease the salinity level while reducing stress and maintaining the PLs' well-being:

Gradual Salinity Reduction: Instead of attempting to drop the salinity level from 25 PPT to 0 PPT in just 7 days, it's advisable to do it gradually over a longer period. Rapid changes in salinity can cause stress and harm the PLs. A recommended approach is to decrease the salinity by 3-5 PPT per day.

Monitor PLs' Behavior: During the salinity reduction process, closely observe the PLs for any signs of stress or abnormal behavior. If you notice increased mortality, lethargy, or abnormal swimming patterns, it might be an indication that the salinity change is too rapid or stressful for the PLs.

Maintain Water Quality: To reduce stress during the salinity reduction, ensure that the water quality parameters remain stable. Monitor and maintain optimal temperature, pH, dissolved oxygen levels, and ammonia/nitrite levels. Any fluctuations in these parameters can further stress the PLs.

Acclimation Tanks: Consider setting up acclimation tanks or compartments within the larval rearing system. These compartments can act as intermediate zones where salinity is gradually reduced. Transfer the PLs to these compartments first and then further decrease the salinity over a couple of days until reaching the desired level.

Calcium and Magnesium Supplementation: Adding calcium carbonate and magnesium chloride can help balance mineral loss during the salinity reduction process. However, it's important to note that the amount of supplementation required may vary depending on the specific water chemistry and the rate of salinity reduction. There isn't a specific formula to determine the exact amounts of calcium and magnesium for each salinity PPT decrease. It's recommended to consult with a marine biologist, aquaculture expert, or water chemistry specialist who can analyze your water parameters and provide tailored recommendations.

Water Testing and Adjustment: Regularly test the water parameters, including calcium, magnesium, alkalinity, and other essential minerals. Adjust the supplementation amounts based on the test results and the observed behavior and health of the PLs. It's crucial to strike a balance between preventing sudden changes in water chemistry and maintaining optimal mineral levels for the PLs' health.

Observing PLs' Response: Throughout the salinity reduction process, carefully observe the PLs' response and adjust the rate of salinity reduction accordingly. If you notice signs of stress or if the PLs are not adapting well, slow down the process and allow them more time to acclimate.

Remember, maintaining the health and well-being of the PLs is of utmost importance. Consult with experts in the field, gather data on the specific water conditions and PLs' responses, and make adjustments based on observed outcomes.

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