Any one recommended best method for genotyping with lower cost ??

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Best novel method to optimize 3D geometry and its fluid?

Do you have any idea what is the best method to optimize 3D geometry and its fluid without linking the code with fluent (or any flow solver) ? In order to find the best 3d geometry and decrease...

10 April 2020 9,321 1 View

I have problem with cytotoxicity test of gemcitabine.how can i fix it?

Hi, i am working on effects of gemcitabine in HepG2 cell line and i should use the IC50 of Gemcitabine in the experiments.i found different values in literature and the numbers are highly variable...

06 December 2019 6,382 3 View

What is the IC50 of Gemcitabine in HepG2 cell line?

Hi, i am working on effects of gemcitabine in HepG2 cell line and i should use the IC50 of Gemcitabine in the experiments.i found different values in literature and the numbers are highly variable...

26 August 2019 8,545 4 View

In flow analysis in FLAC software, what is the reason for moving the model after the flow analysis in the earth dam?

Hello I have a model of earth dam in FLAC 2D and I did an uncoupled analysis for mechanical and flow. (set mech off/flow on ) Next step, when mech on/flow off ,the model starts to move in the...

14 July 2019 5,146 3 View

Do any researcher have ideas for the best medical Phantom that could be employed in segmating 3D medical data?

I am doing a research for segmenting 3D medical volumes using different techniques I used recently NEMA IEC body phantom and CIRS abdominal phantom,model 057 in my research I am looking for the...

19 May 2019 9,979 2 View

I have problem with designing a primer for my gene,how can i fix it???

Dear all.Hi. i want to design a primer for gene(BCl2) that have multiple variant. And I can not design a primer that includes all my gene variants and also be specific for my gene. what should i...

13 January 2019 2,430 2 View

How can i clone a plasmid?

how can i clone a plasmid that i don't know any thing about its sequence , and the backbone of the plasmid?

05 January 2019 6,028 15 View

Is degenerate primers appropriate for real time pcr?

hi... can I use degenerate primers for real-time? I have some genes related to the enzyme in the root but these genes are gene families .and I don"t know which gene controlled my desire enzyme,...

20 December 2018 3,452 2 View

Why do i have band in negative control in ITS primer?

I look for variation in strain bacterium and I want to do sequencing but I have a band in negative control that same in my strain bacterium first time I noticed band in my negative control, I...

02 December 2018 5,889 10 View

The data of maximum specific growth rate in the continuous biorector versus pH?

Hello Dear all I want to examine the effect of pH on maximum specific growth rate in the continuous biorector for acetic acid production by Acetogenium kivui or Clostridium thermolacticum...

17 October 2017 9,311 1 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration fors?

Warm greetings My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology (on Human papillomavirus). At this stage, I have almost completed a clinical sample Cervical swabs)...

25 February 2021 1,660 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration forms?

Warm greetings! My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology focusing on Human papillomavirus). At this stage, I have almost completed a clinical sample (Cervical swabs)...

25 February 2021 3,612 3 View

Errors in DNA sequences?

I am trying to better understand the scope of DNA replication and sequencing errors, e.g. 1. I have seen similar error rates of 10e4 to 10e5 for cell & instrumental DNA replication,...

24 February 2021 4,397 3 View

What might be the reason for obtaining two bands in plant genomic DNA extraction?

I got a thin band and a thick band i guess it would be the genomic DNA and the 260/280 ratio is 3.

21 February 2021 6,523 6 View

I can detect my protein but the encoding DNA sequence has stop codons within. Should I ignore the stop codons?

I constructed a synthetic DNA library (scFv, VH-VL orientation) with a 3' reporter and 8x histag. I cloned and expressed this gene following which I performed an ELISA. The ELISA results suggested...

19 February 2021 7,006 5 View