25 September 2014 3 274 Report

I need to generate Fab and Fc fragment with Papain and Fab' with pepsin to check the interaction with my ligand. I need to know the protocol for the digestion with papain and pepsin?

More Tushar Savane's questions See All
1. How to remove bubble of agarose gel or after prepared it?

1. How to remove bubbles during preparation of agarose gel or after prepared it? 2. When we inject the gel inside the capillary tube, there are some bubbles visible.what is the way to remove...

22 September 2023 2,821 0 View

What is the molecular identity and function of Endothelial Barrier antigen (EBA)?

First reported in 1987, the Endothelial Barrier Antigen (EBA) is expressed in brain endothelium and is downregulated in ischemic stroke. Its expression is negatively correlated with BBB...

30 August 2023 5,648 1 View

Is it possible to assess BBB permeability in mice using intraperitoneal injection of tracers?

Fluorescent tracers (sodium fluorescein and Dextrans) are usually injected into the lateral tail vein of the mouse. The tracers are allowed to circulate for 30 minutes before trans cardiac...

13 August 2023 7,782 0 View

Does a middle cerebral artery penetrate the skull before entering brain parenchyma?

MCA and other arteries coming from circle of Willis arise from the base of the brain (ventral mouse brain). Do they travel along the skull bone, then penetrate the bone and underlying meninges...

23 April 2023 9,123 2 View

Why polyquaternium 7 gives haze or precipitation in SLES based formulation ?

For my research purpose i have prepared polyquaternium 7 with 41 - 45 % solids and viscosity in the range of 2000 - 45000 using 70 % acrylamide and 30 mol % DADMAC polymer solution itself is...

10 April 2023 1,639 1 View

What are the possible reason ?

Can any one tell me why the flip was there in my 100 ns MD simulation where the red one experimental and black one is control.

21 March 2023 112 2 View

Mechanism of Pb availability as affected by hardness of water?

hello respected scholars, i am looking for research and chapters (if available) detailing the impacts of irrigation water quality on the plant availability of Pb and other heavy metals in soil. i...

16 January 2023 9,301 2 View

I am getting Raman peaks for my graphene quantum dot sample which doesnot seem to be like the typical raman shifts observed incase of GQDs reasons ?

Laser-514 nm grating-1800 power-0.1

06 January 2022 2,969 6 View

How to determine assay of Ammonium Bicarbonate by Titration?

Looking for detailed Titration method for the quantitative determination of Assay of Ammonium Bicarbonate. I am having one titration method, which includes the heating of solution before...

08 December 2021 3,308 7 View

How to find differences between single cell RNA seq samples beyond cluster information and differential expression?

Consider a single cell RNA seq data with a control and a test sample. These are integrated to minimize the batch effect. Reads have been normalized to the sequencing depth. One can compare the...

22 September 2021 1,874 3 View

Similar questions and discussions
Why does my protein refolded to beta sheet during thermal denaturation analysis?

Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...

06 August 2024 1,989 3 View

Low-yield gel extraction problem?

I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...

05 August 2024 9,798 3 View

Transfection in HEK293T cells?

Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...

31 July 2024 9,892 4 View

How to retain the a GFP tagged gene expression in stable cell line?

Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....

29 July 2024 5,007 0 View

What is the relationship between protein structure and N or C terminal tagging choosing?

I want to do 2,3-butanediol dehydrogenase(BDH) enzyme purification to confirm its activity for 2,3-butanediol. Before that, I need to confirm which N or C terminal tagging is better for enzyme...

28 July 2024 366 3 View

How to conduct a sensitivity power analysis for Kendall's Tau?

Is there a straightforward way to conduct a sensitivity power analysis for a Kendall's Tau correlation? I was considering using the sensitivity setting and "Correlation: point biserial model" test...

28 July 2024 6,133 8 View

Why cannot i find my protein on cell surface after antibiotic selection of expressing plasmid?

I cannot confirm cell surface protein expression by flow cytometry even after transfection and antibiotic selection of cells. Does it take long for proteins to express on the cell surface? the...

28 July 2024 3,178 3 View

How does the Retention time effect the Biogas Production?

List down the various factors responsible and how does this effect the overall biogas production when it is related to Hydraulic retention time of the digester based on the feedstock?

27 July 2024 4,800 4 View

Can anyone help me with in providing the procedure for purification of GSTtagged protein by gst affinity chromatography without an automated machine?

Purification of gst tagged protein

25 July 2024 1,720 0 View

EDC carbodiimide and NHS easter 's half life among different pH?

It has been long known that EDC carbodiimide and NHS easter's half life agains hydrolysis are highly related to pH. However, very surprisingly, not yet found a paper giving a table/chart of their...

25 July 2024 8,738 0 View

Our partners will collect data and use cookies for ad personalization and measurement. Learn how we and our ad partner Google, collect and use data. Agree & close