Any one has experience in In vivo lung metastasis by I.P injection of cancer cells?

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Do you have any proposal in public health?

please send I would like to make one but need a sample

01 January 2021 1,176 11 View

What is the reason for getting noise in SEM image from a metal surface?

I failed to get a clear image from the surface of copper by SEM. the noise gets stronger as I decrease the scan speed or higher magnifications

03 November 2020 8,100 5 View

How can I remove the background signal of graphene/SiO2 Raman spectra in Origin?

it is CVD graphene on SiO2 substrate.

30 April 2019 4,847 8 View

Any one has experience in In vivo lung metastasis by I.P injection of cancer cells?

I was wondering if I.P route could rise Lung metastasis as I.V in mice tumor models. Any one with experience about it to share would be very much appreciated.

10 January 2019 314 2 View

Analytical method development help?

I am using Indirect ELISA to determine the concentration of my antibody and generate standard curve, however this method cant pick up concentration differences precisely. is there any other...

21 May 2016 2,292 17 View

Can anyone advise me about RIP-Chip with anti-miR in tumor cells?

Hi All.. I was wondering if you have an antimiR that has an effect on its correspoding miR.. would it be obvious to see this effect also in AGO2 complex in RIP-Chip? I got some enrichment of my...

18 February 2015 8,116 2 View

Can anyone advise me about RIP-Chip with anti-miR in tumor cells?

Hi All.. I was wondering if you have an antimiR that has an effect on its correspoding miR.. would it be obvious to see this effect also in AGO2 complex in RIP-Chip? I got some enrichment of my...

01 February 2015 8,152 2 View

How can I find my miRNA amplicon size?

I have designed MicroRNA forward specific primer which I have used along with universal reverse primer in my QPCR reaction. Is there any web based tool which I can get the amplicon size in a...

03 April 2014 3,233 2 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

What analysis to choose with a large neste dataset and clearly skewed or kurtotic distributions?

Hello! I have a dataset of n=3000 nested within 8 countries with approximately 200 or 400 responses in each country. I originally planned to perform multilevel modelling with 4 dependent variables...

02 March 2021 6,865 1 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

How to avoid ulcers in s.c. injected tumors ?

Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...

01 March 2021 9,938 3 View