Lipid structures disappear upon negative stainging. Does anyone knows this effect?

13 December 2020 1,559 3 View

Cryo-EM Vitrobot settings for large lipid structures. How can I reduce ice thicknes?

Dear all, I have an issue figuring out good plunging conditions for large lipid structures: I have carbon coated C-Flat 2/2 400mesh copper grids with large (elongated) lipid structures on it....

13 December 2020 6,696 2 View

Can I use lentiviral packaging HEK293T cell lines to produce stable clones?

Hi, I generally made LV in HEK293T cell lines by triple transfection. I am wondering whether the newly generated LV will transduce the packaging HEK293T. In this way I could exploit the...

09 September 2020 6,333 3 View

Which software is the best for scientific presentations in which equations occupy a big part of it?

I usually use Beamer, in which equations are easy to write (and I already have them in Latex) but it's too static and rigid. In Powerpoint it is impossible to write the equations, it takes a lot...

15 July 2020 8,260 3 View

How to calculate the max Jsc for a given Eg using spectral irradiance [W/(m2*eV)] data?

I'm calculating the max available Jsc as a function of bandag Eg. For that I'm using the following integral: Jsc=q*int(f(Eph)*EQE.dEph) from Eg to \inf With: q=1C/eV f(Eph) Spectral Irradiance...

07 July 2020 5,873 4 View

Inverted current Mirror connection in LM741?

Analyzing the topology of the LM741 , the current mirror (Q8-Q9) that bias the first stage of the op. amp. is not connected as usual (see the red circle on the picture). Curiosly, this way to...

24 January 2020 8,419 2 View

Negative values in anti-inflammatory assay: inhibition of albumin denaturation?

I am performing in vitro anti-inflammatory assay: inhibiton of albumin denaturation by organic extract and purified fractions from natural material. Since my samples are colored (red, orange,...

14 September 2019 2,528 1 View

Can you help me with Python script?

Hi to all, I have an approaching deadline and to perform my analysis (design guide RNAs) I need to insert a lot of mutations in my sequenze (txt file). Mutations are inside a excel in file...

05 February 2019 2,494 1 View

What are good color palettes for students in the autism spectrum?

I've read that, for students in the spectrum, it may help to design a class environment that avoids overstimulating their cognitive system with unnecessary distractions, one of them begin colorful...

28 January 2019 2,557 3 View

How to improve undergraduate and postgraduate educational settings with gamification?

Gamification is a brilliant strategy to support the development of educational settings, especially in undergraduate and postgraduate courses. Therefore, I would like to ask you what do you think...

22 October 2018 2,456 3 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View