Any issues that would arise if I used 5X higher the concentration of live/dead stain?

12 December 2016 1 6K Report

I am thinking it would not matter for my analysis since live cells would not be stained with the live/dead stainining reagent....am I wrong?

Kerstin Andersson

Hi Sharon,

I would assume that you are talking about Flow Cytometry?

There is always some unspecific binding of most reagents. I usually get an even better result (better separation between live and dead) if I dilute even more than recommended by the supplier. Some viability stains can also be toxic to cells in a higher concentration.

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