Any input on the use of serum-free media to culture monocytes or HepG2 cell line for (only) 24h (as opposed to using media with 2% human serum)?

10 October 2016 1 7K Report

Particularly, the media is AIM-V (see link) by Gibco, which the company claims can be used without serum to culture certain cell types. https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/specialty-media/t-cell-media/aim-v-medium.html

Ke-Wei Zhao

Sharon: HepG2 cells can be grown in serum-free Williams E medium supplemented with Hepa (500 x concentrate, both from Invitrogen ) for more than 24 hrs without any obvious changes. Good luck.

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