Is there an association between SPS phenotype and genetic abnormalities?
I have a time series data of some biochemical studies ( DNA and Chromosomal damage) which I intend to use to further predict into future without necessarily conducting the assay for an extended...
23 February 2021 7,842 1 View
Hello, i am a bit curious about my results. I transfected the human cell Line ARPE-19 with a Plasmid containing a GFP tagged p53 (p53-GFP, Plasmid #11770). After 24h i checked under the...
18 February 2021 3,811 7 View
Am searching for a stand-alone permanently in-ground soil-moisture sensor with an installed GSM module, for outdoor use. The idea is for the sensor to send a moisture reading via a cellphone text...
04 February 2021 2,047 4 View
Hello everyone! I have one question. I have couple of solvents and all of them were pure when they were bought but after some months or years of storage after opening I am not 100% sure about...
03 February 2021 2,154 8 View
Hi I want to set up a virus neutralisation assay such as PRNT or FRNT at my lab (BSL2), but im uncertain of the bio safety requirements that has to be fulfilled in order to work with...
01 February 2021 5,626 3 View
I'm trying to use LINK11 to apply a force as if it was a cable pulling a structure twowards a point in ANSYS APDL. When I solve the model, the deformation of the overall structure is far too...
30 January 2021 7,919 1 View
We have been having clotting issues with our Cytoflex S from Beckman Coulter. Back in December 2020, we had to have the tubing system and sample needles replaced, but we are beginning to observe...
28 January 2021 6,897 2 View
I'm currently trying to purify Outer Membrane Vesicles (OMVs) following their extraction from the bacteria I'm working with. After the initial extraction the concentration of protein seems good...
27 January 2021 6,852 3 View
Most of us use tooth paste more times a day. The empty tube we throw away. I wondered how empty the tube really is. This does not sound being Research? I began to collect empty tubes...
19 January 2021 808 3 View
We all use face masks many hours a day. I wonder what are your items you like to be improved. We have the opportunity to write an application for support to a sponsor. Your answers will help us a...
18 January 2021 1,311 4 View
Hi, I encounter some problems, I need to my research to fix and cryoprotec some blood clot for histology analyses. Mys current protocol is the following : 48h of para formaldehyde 4% and 7 days of...
07 February 2021 3,756 3 View
As it is well known fact that nano-particles can easily aggregate during nucleation/ synthesis part due to high surface energy..by reading literature, I came to know that surfactants can help to...
30 January 2021 8,206 6 View
Hello everybody. Im new to STATA and this forum but I hope you can help me. I am doing a research project where I intend to estimate the prevalence of venous thromboembolic events (vte) up to 30...
24 January 2021 665 5 View
Hi, I have overall weekly scores for 2 executives for 6 weeks and would like to make some comparison based on this limited dataset. I know the dataset needs to be improved but currently, this is...
02 January 2021 6,091 3 View
The SARS-CoV-2 virus spreading across the world has led to surges of COVID-19 illness, hospitalizations, and death. The complex and multifaceted pathophysiology of life-threatening COVID-19...
29 December 2020 7,446 4 View
The protein sample was analyzed after dropping. The buffer had NaCl in it. I just know that something that looks black might be air bubbles. But here these particles are elongated. So I couldn't...
07 December 2020 5,097 2 View
Dear Concern, Kindly guide me regarding the step by step process of data aggregation in Mplus/SPSS. (Pls answer as per your expertise in any of the mentioned statistical software). I have...
27 October 2020 8,554 3 View
I want to separate DNA from human old clotted blood . I haven't any idea how to extract DNA from it. Your kind expertise and thought would be appreciated.
07 October 2020 7,871 2 View
Hi All, Recently, I purified 8 proteins and stored at -20 with the elution buffer containing 20 mM Tris, 250 mM NaCl and 200 mM Imidazole. I autoclaved the buffer, so I do not know whether the...
25 September 2020 9,814 6 View
Hi, Does anyone have a method for counting platelets on flow cytometry (cytometer=Cytoflex) without the need of using TRUcount beads or reference beads? Is it possible or do I need to purchase...
23 September 2020 3,509 1 View