Any hints on the number of B and T cells from adipose tissue?

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Which test should be used to study association among demographic profile and awarness level?

i have to study the awareness and adoption level of cloud computing in a district of India. i also want to use association among demographic variables like gender, age, education, income etc and...

02 August 2024 2,420 3 View

How can we recover Iron or acid from industrial waste water, pH of waste water is approximately 2 ?

Steel processing industry releases waste water, which contains 20% of Iron with 2 pH. can you suggest any low-cost technique to separate or recover anyone, either acid or Fe from the waste water, pls

24 April 2024 5,636 6 View

How to generate intermidiate replicas (with kinks) using linear interpolation for NEB calculations LAMMPS?

I am interested to calculate the peierls barrier for the movement of screw dislocation in BCC iron between two peierls vally. For this I am using nudged elastic band (NEB) method in LAMMPS. We...

15 April 2024 4,794 1 View

Why do microbiota composition results from MOTHUR and CLC Genomics workbench pipeline differ so much, even with the same reference database?

Our samples had been analysed by Mothur + Silva 119 reference DB around 2015. When analysing the same samples in 2023 with CLC genomics workbench and SILVA 119 DB, the results are strikingly...

21 March 2024 991 1 View

During normal inhalation, pressure in alveoli is 3 mmHg below atmospheric pressure, enabling air to flow into them. How is this pressure achieved?

For a better teaching in the class.

19 February 2024 274 1 View

What could be the reason for no expression of protein after transfection in HEK293 cells?

I have cloned my genes in a CMV vector and transfected them in HEK293 cells. After harvesting the cells, the expression level at the transcriptional level is increased compared to the control...

23 December 2023 2,507 10 View

During storage of Ethanol (USP Grage) particulate matter observed. Is there any science behind these particulate matter?

During storage of Ethanol (USP Grage) after filtration through 5-micron filter membrane. Particulate matter observed during storage. For that, we need to investigate the reason. Is any science...

26 November 2023 2,540 3 View

Struggling for purification process development related to product analog impurity. Need help to resolve issue ?

In one of our pregnane ring structure related product (Drospirenone), we are facing one impurity (i.e. 6-Methyl Drospirenone impurity) problem. The observed level of impurity is about 1% on USP...

25 November 2023 5,102 16 View

How can i form the schematic diagram of g quadruplex as shown in figure?

how can i form the schematic diagram of g quadruplex as shown in figure?

24 November 2023 1,851 1 View

What tools can be used to make Double Kink dislocation in BCC alloy?

We are working on kink nucleation and migration using NEB method in LAMMPS. To provide intermediate replicas we need to make kink pairs. Can anyone suggest a method or tool that can be used to...

19 November 2023 8,304 4 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Microtome tissue sectioning - How to prevent chatter of paraffin tissue specimen sections?

Hello everyone, I have recently started using the microtome device for sectioning of paraffin-embedded mice lung. While I had some success in sectioning and observing proper ribbons, some of my...

06 August 2024 666 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

The Curse of Evolution and Complexity?

Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...

05 August 2024 6,247 3 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View