Hi all,

I've been trying to get an IF for neutrophils (using an anti-CD66b antibody) in FFPE liver tissue sections to work, but no success so far. The protocol is run on a Ventana automated system. I got the same antibody to work perfectly on IHC in the same kind of tissue sections, but I've been having issues when trying IF, even though the tissue goes through the same antigen retrieval process as for IHC. Any input as to what might be going wrong for the IF that prevents me from getting specific staining?

Thank you!

Joao

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