Hi Joao, I encountered the same problem recently. I contacted with the FlowJo support team and they told me that version 10 has some problem with their cell cycle analysis platform. However, they recommend me to use version 7.6.5 where you can apply both Watson (pragmatic) and Dean Jett Fox model.

Hi Ruhul! Thank you so much for your help! I will then use version 7.6.5. However, I'm afraid I'll have to redo the analysis, since the original file is from version 10, right?

Hi Joao, I think the Watson model is the one that is automatically applied in version 10 but you can't switch between models like in previous versions. So if you want to use the Dean Jett Fox model I guess you need to use version 7.6.5.

I don't know what was the type of your input file (FCS/LMD) but I think it won't take too long to redo the analysis in earlier version.

Best wishes.

I never used v10 for Cell cycle analysis. I did all my cell cycle on v8.7

Thanks for the info on v10 bug.

Is there a good paper/link/tutorial on which model is better and how to modify parameters to fit the models to the data?

hi all,

i have a question on the same lines. is it advisable if i apply watson model to one sample and DeanJett Fox model to other sample and can still compare them? Watson model is not giving me any output for one same and i can apply Deanjett fox model to both of them. but, still i would like to know if i can use different model for different samples and still compare them.

Thanks a lot in advance

Amit

Hi Amit,

You would need to apply the same model to all samples as they use different algorithms to fit the data. If you want to use the Watson model, you could try changing the parameters to fit the data but again, you would need to apply these changes to all samples so that they are comparable.

The FlowJo website tutorials includes how you can change the parameters to fit the data if you need help with it.

Hope this helps,

Anita

Could anyone please let me know, how I can adjust the Y axis (similar for all histogram) in cell cycle analysis by FlowJo v7.6.5 .

Does anyone know how to apply the Dean Jett Fox algorithm without the use of special softwares? I have attempted it using excel, but I think its full of flaws. I don't think I have applied it correctly. Has anyone tried to use these algorithms from the original papers by Dean and Jett or Watts?