João Maurício

5 Questions 9 Answers 0 Followers

Questions related from João Maurício

Any help with neutrophil IF in FFPE liver tissue?

Hi all, I've been trying to get an IF for neutrophils (using an anti-CD66b antibody) in FFPE liver tissue sections to work, but no success so far. The protocol is run on a Ventana automated...

06 June 2019 1,745 4 View

Antibody for IL-17 detection using IHC in mouse liver samples?

Hi everyone! Does anyone know of an antibody anti-IL-17 for IHC in mouse liver tissue (FFPE)? I'm trying to detect Th17 cells, but every paper I can find deals only with human cases. Thanks in...

02 February 2017 3,274 3 View

Differentiation of THP-1 cells and evaluation of CD11b expression?

Hi everyone! I've been trying to optimise a CD11b antibody, using THP-1 cells. In the latest experiments, I've spiked whole blood from healthy donors with differentiated THP-1 cells, but I get no...

11 November 2016 4,814 8 View

How to use mathematical models for cell cycle, on FlowJo v.10?

Hi everyone! Can someone tell me how to apply mathematical models (Watson model) to cell cycle analysis, on FlowJo v.10 (trial version)? Thank you so much for your help!

08 August 2014 3,931 10 View

Can caspase-9 be activated without occuring mitochondrial membrane depolarization?

During my master's dissertation work, studying the biological effects of new potential aromatase inhibitors in hormone-dependent breast cancer (MCF-7aro cells), I observed that the compounds...

08 August 2014 591 9 View